Single-molecule enzymology: fluorescence-based and high-throughput methods

by Chemla, Yann R. Spies, Maria

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Title Statements

Full title :Single-molecule enzymology : fluorescence-based and high-throughput methods / edited by Maria Spies, University of Iowa, Iowa City, IA, United States, Yann R. Chemla, University of Illinois at Urbana-Champaign, Urbana, IL, United States.

Edition Statements

Publication Date:2016.
Edition:First edition

Publication Statements

Publisher(s):Academic Press/Elsevier
Place of Publication:Amsterdam
Language:eng
Publication Date:2016.

The Physical Object

Description:1 online resource (xviii, 598 pages :) : illustrations (some color).

Edition Identifiers

ISBN:9780128092675, 012809267X
oclc:on1235834786

Chapter 1. Direct fluorescent imaging of translocation and unwinding by individual DNA helicases -- Chapter 2. Single-molecule imaging with one color fluorescence -- Chapter 3. Measuring membrane protein dimerization in lipid bilayers by single-molecule fluorescence microscopy -- Chapter 4. Fluorescent labeling of proteins in whole cell extracts for single-molecule imaging -- Chapter 5. Quantifying the assembly of multicomponent molecular machines by single-molecule total internal reflection fluorescence microscopy -- Chapter 6. How to measure separations and angles between intramolecular fluorescent markers -- Chapter 7. Precisely and accurately inferring single-molecule rate constants -- Chapter 8. Quantification of functional dynamics of membrane proteins reconstituted in nanodiscs membranes by single turnover functional readout -- Chapter 9. Putting Humpty-Dumpty together: clustering the functional dynamics of single biomolecular machines such as the spliceosome -- Chapter 10. Single-molecule FRET to measure conformational dynamics of DNA mismatch repair proteins -- Chapter 11. Single-molecule confocal FRET microscopy to dissect conformational changes in the catalytic cycle of…

Subjects

Cinétique enzymatique Conference papers and proceedings Enzyme kinetics Enzymes -- Analysis fluorescence microscopy Fluorescence Resonance Energy Transfer Fluorescence spectroscopy Fluorescence spectroscopy -- Congresses Fluorescent Dyes -- analysis Fluorescent probes Fluorescent probes -- Congresses Kinetics Luminescent probes Luminescent probes -- Congresses Microscopie de fluorescence Microscopy, Fluorescence Sondes fluorescentes -- Congrès Sondes luminescentes -- Congrès Spectrometry, Fluorescence Spectroscopie de fluorescence