Single molecule enzymology: methods and protocols

by Batters, Christopher Mashanov, Gregory I.

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Title Statements

Full title :Single molecule enzymology : methods and protocols / edited by Gregory I. Mashanov, Christopher Batters.

Summary

The last fifteen years have witnessed the birth and maturation of many original methods and the development of protocols specific to single molecule measurements and their analysis, including techniques involving optical imaging, electron microscopy, optical and magnetic trapping, and developments in atomic force microscopy. In Single Molecule Enzymology: Methods and Protocols, experts in the field provide procedures which enable the extraction of detailed information about enzyme work cycles, their static and kinetic properties, and information about their location and activity within cells. The detailed volume offers practical advice on many aspects of single molecule enzymology and includes strategic overviews of interconnected methods involved in sample preparation, single molecule measurements, and data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Single Molecule Enzymology: Methods and Protocols is intended for use within the diverse community of molecular biologists, biochemists, and biophysicists studying enzymes in detail and can be used by researchers planning their first single molecule study or to aid more experienced researchers in further developing their existing studies.

Edition Statements

Publication Date:©2011.

Publication Statements

Publisher(s):Humana, Springer
Place of Publication:New York
Language:eng
Publication Date:©2011.

The Physical Object

Description:1 online resource (xi, 294 pages) : : illustrations (some color)

Edition Identifiers

ISBN:9781617792618, 1617792616, 9781617792601, 1617792608
DOI:10.1007/978-1-61779-261-8
oclc:ocn747105612
LC Classification:QP601
Dewey Decimal:572/.7

Subjects

Biochemical Phenomena Biochemie biochemistry Chemical Phenomena Chemicals and Drugs Chemistry Chimie Disciplines and Occupations Eiwitten en enzymen enzyme Enzymes Enzymologie Enzymology Internet resources Kinetics Laboratory manuals Mechanical Phenomena Metabolism metabolism (biological concept) Métabolisme