Stem cell manufacturing

<P>1. Genetic Engineering in Stem Cell Manufacturing<i></p></i> <p>2. Biomechanics in Stem Cell Manufacturing<i></p></i> <p>3. Bioreactor Engineering Fundamentals for Stem Cell Manufacturing<i></p></i> <p>4. Microcarrier Culture Systems for Stem Cell Manufacturing<i></p></i> <p>5. Novel Single-Use Bioreactors for Scale up of Anchorage-Dependent Cell Manufacturing for Cell Therapies<i></p></i> <p>6. Bioreactors and Downstream Processing for Stem Cell Manufacturing<i></p></i> <p>7. Cell production system based on flexible modular platform<i></p></i> <p>8. Microfluidic Devices for the Culture of Stem Cells</p><i> <p></i>9. Enrichment and Separation Technologies for Stem Cell-Based Therapies<i> </p></i> <p>10. Expansion and Characterization Considerations for the Manufacturing of Stem Cells</p><i> <p></i>11. Storage and Delivery of Stem Cells for Cellular Therapies<i></p></i> <p>12. Business Models for Manufacture of Cellular Therapies<i></p></i> <p>13. Stem cells for the regeneration of chronic wounds: a translational perspective<i></p></i>

Front Cover; STEM CELL MANUFACTURING; STEM CELL MANUFACTURING; Copyright; CONTENTS; LIST OF CONTRIBUTORS; INTRODUCTION; 1 -- Genetic Engineering in Stem Cell Biomanufacturing ; 1.1 INTRODUCTION; 1.2 GENETIC MANIPULATION APPROACHES IN HUMAN PLURIPOTENT STEM CELLS; 1.2.1 Transgenic Approaches; 1.2.2 Knock-In and Knock-Out Approaches; 1.2.2.1 Zinc-Finger Nucleases; 1.2.2.2 Transcription Activator-Like Effector Nucleases; 1.2.2.3 Clustered Regularly Interspaced Short Palindromic Repeat/Cas9; 1.2.3 Bacterial Artificial Chromosomes; 1.3 APPLICATIONS

1.3.1 Genetic Labeling for Cell Identification and Cell Tracking1.3.2 Gene Alteration for Directed Differentiation; 1.3.3 Gene Disruption for Functional Investigation; 1.3.4 Gene Correction for Function Restoration; 1.4 DELIVERY METHODS; 1.4.1 Transfection; 1.4.2 Nucleofection; 1.4.3 Viral Transduction; 1.5 CONCLUSIONS; ACKNOWLEDGMENTS; REFERENCES; 2 -- Biomechanics in Stem Cell Manufacturing; 2.1 INTRODUCTION; 2.2 CELLULAR BIOMECHANICS; 2.2.1 Biomechanical Cues; 2.2.2 Shear Forces and Differentiated Cells; 2.2.3 Shear Forces and Pluripotent Stem Cells

2.3 SCALE UP TOWARD PRODUCTION-LEVEL BIOREACTORS2.4 BIOMANUFACTURING CELLS FOR THERAPIES…