TGF-beta signaling: methods and protocols

by Liu, Xuedong Zi, Zhike

Type Book

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Title Statements

Full title :TGF-beta signaling : methods and protocols / edited by Zhike Zi, Xuedong Liu.

Summary

This detailed volume is devoted to the recent development of quantitative experiments and computational methods driving new transforming growth factor beta (TGF-beta) and other cell signaling knowledge. Many chapters cover quantitative assays for TGF-beta signaling studies, with others exploring the increasing role of both modeling and computational methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, TGF-Beta Signaling: Methods and Protocols serves as a vital resource for researchers seeking to move the TGF-beta field into the quantitative realm.

Edition Statements

Publication Date:2022

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:2022

The Physical Object

Description:x, 239 pages : : illustrations (some color) ; : 26 cm.

Edition Identifiers

ISBN:9781071622766, 1071622765
oclc:on1287919609
LC Classification:QP552.T72
Dewey Decimal:572.65

Absolute Quantification of TGF-[beta] Signaling Proteins Using Quantitative Western Blot -- Fast Quantitation of TGF-[beta] Signaling Using Adenoviral Reporter -- Complex Formation among TGF-b Receptors in Live Cell Membranes Measured by Patch-FRAP -- Branched Proximity Hybridization Assay for the Quantification of Nanoscale Protein-Protein Proximity -- Visualizing Dynamic Changes during TGF-[beta]-Induced Epithelial to Mesenchymal Transition -- Establishment of Embryonic Zebrafish Xenograft Assays to Investigate TGF-[beta] Family Signaling in Human Breast Cancer Progression -- Generating Somatic Knockout Cell Lines with CRISPR-Cas9 Technology to Investigate SMAD Signaling -- CRISPR-Based Screening in Three-Dimensional Organoid Cultures to Identify TGF-[beta] Pathway Regulators -- Optogenetic Control of TGF-[beta] Signaling -- Using Microfluidics and Live Cell Reporters to Dissect the Dynamics of TGF-[beta] Signaling in Mouse Embryonic Stem Cells -- Energy Landscape Analysis of the Epithelial-Mesenchymal Transition Network -- Discrete Logic Modeling of Cell Signaling Pathways -- Mining of Single-Cell Signaling Time-Series for Dynamic Phenotypes with Clustering -- Automated Classif…