Title Statements
- Full title :The ESCRT Complexes : Methods and Protocols.
Edition Statements
- Publication Date:2019.
Publication Statements
- Publisher(s):Springer
- Place of Publication:New York, NY
- Language:eng
- Publication Date:2019.
Notes
3.4.2 Fluorescent Microscopy Using Inversed Microscope (Fig. 5c and d)
The Physical Object
- Description:1 online resource (327 pages)
Edition Identifiers
- ISBN:9781493994922, 1493994921
- oclc:on1122919474
- LC Classification:QH301-705
Table of Contents
Intro; Preface; Contents; Contributors; Chapter 1: Analysis of the Archaeal ESCRT Apparatus; 1 Introduction; 2 Materials; 2.1 Cell Culturing; 2.2 Baby Machine Synchronization; 2.3 Immunofluorescence Microscopy; 3 Methods; 3.1 Growth of Sulfolobus acidocaldarius; 3.2 Baby Machine; 3.3 Immunofluorescence Microscopy; 4 Notes; References; Chapter 2: Genetic and Cell Biology Methods to Study ESCRTs in Drosophila melanogaster; 1 Introduction; 2 Materials; 2.1 Dissection; 2.2 Notch Uptake Assay; 2.3 Immunohistochemistry; 2.4 Mounting; 3 Methods; 3.1 Dissection of Larval Eye-Antennal Imaginal Discs
3.2 Notch Uptake Assay3.2.1 Notch Antibody Incubation; 3.2.2 Fixation 0 min Time Point; 3.2.3 Notch Uptake; 3.2.4 Fixation 15 min Time Point; 3.2.5 Fixation 45 min Time Point; 3.2.6 Fixation 4 h Time Point; 3.3 Immunohistochemistry; 3.3.1 Fixation; 3.3.2 Blocking; 3.3.3 Antibody Incubation; 3.4 Mounting; 3.4.1 Mounting Eye-Antennal Discs; 3.4.2 Sealing of the Slide; 4 Notes; References; Chapter 3: Functional Analysis of ESCRT-Positive Extracellular Vesicles in the Drosophila Wing Imaginal Disc; 1 Introduction; 2 Materials; 2.1 Genetic Identification of ESCRT Genes Required for Hh Secretion
2.…
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