Theranostics: Methods and Protocols

by Batra, Jyotsna

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Title Statements

Full title :Theranostics : Methods and Protocols.

Summary

This volume explores disease diagnosis and therapy in developing theranostics. The chapters in this book are divided into four parts and cover various techniques used in studying bioengineering, molecular diagnostics, in vivo imaging, and imaging-guided therapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Theranostics: Methods and Protocols is a valuable tool for novice and expert clinicians, researchers, and students working in this multidisciplinary field.

Edition Statements

Publication Date:2019.

Publication Statements

Publisher(s):Springer
Place of Publication:New York, NY
Language:eng
Publication Date:2019.

Notes

3.1 Human Osteoblast-Derived Bone-Like Microtissue (hOBMT)

The Physical Object

Description:1 online resource (294 pages)

Edition Identifiers

ISBN:9781493997695, 1493997696
DOI:10.1007/978-1-4939-9769-5.
oclc:on1122919758
LC Classification:QH506
Dewey Decimal:611.01816

Intro; Preface; Contents; Contributors; Part I: Bioengineering; Chapter 1: Cell Recovery of Hydrogel-Encapsulated Cells for Molecular Analysis; 1 Introduction; 2 Materials; 2.1 Analysis of Cell Viability and Microscopy; 2.2 Analysis of Metabolic Activity; 2.3 Analysis of Mechanical Properties; 2.4 Hydrogel Digestion; 2.5 Flow Cytometry; 3 Methods; 3.1 Analysis of Cell Viability and Microscopy; 3.2 Analysis of Metabolic Activity; 3.3 Analysis of Mechanical Properties; 3.4 Hydrogel Digestion; 3.5 Flow Cytometry; 4 Notes; References

Chapter 2: Bioengineered Microtissue Models of the Human Bone Metastatic Microenvironment: A Novel In Vitro Theranostics Platf ... 1 Introduction; 2 Materials; 2.1 Scaffold Manufacturing; 2.1.1 Materials and Consumables; 2.1.2 Equipment; 2.2 Calcium Phosphate Coating; 2.2.1 Materials and Consumables; 2.2.2 Equipment; 2.2.3 Reagents; 2.3 In Vitro Cell Culture; 2.3.1 Materials and Consumables; 2.3.2 Equipment; 2.3.3 Reagents; 2.4 Human Preosteoblast (hPOB) Isolation and Expansion; 2.4.1 Materials and Consumables; 2.4.2 Equipment; 2.4.3 Reagents; 2.5 hPOB Culture on CaP-mPCL Scaffolds

2.5.1 Materials and Consumables2.5.2 Equipment; 2.5.3 Reagents; 2.6 Biol…