Therapeutic oligonucleotides: methods and protocols

by Goodchild, John,, chemist

Type Book

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Title Statements

Full title :Therapeutic oligonucleotides : methods and protocols / edited by John Goodchild.

Summary

The field of oligonucleotide therapeutics research is ripe with the prospect of new discoveries. In Therapeutic Oligonucleotides: Methods and Protocols, a selection of established and emerging methods for the application of oligonucleotides as therapeutics are presented, all providing the tools needed to inspire great changes in the field. Divided into twenty-one chapters, this detailed volume meticulously describes vital protocols for optimizing and improving cell uptake, such as photochemical internalization, modified cell penetrating peptides, antibody conjugates, and nanoparticles. Other chapters address quantitation of RNA therapeutics in cells, assaying gene knockdown, selecting the best target site and synthesis of various modified oligonucleotides. Written in the successful Methods in Molecular Biology"!series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Therapeutic Oligonucleotides: Methods and Protocols serves as a timely resource for both professionals and novices pursuing research in this exciting and pioneering field.

Edition Statements

Publication Date:©2011.

Publication Statements

Publisher(s):Humana Press, Springer
Place of Publication:New York
Language:eng
Publication Date:©2011.

The Physical Object

Description:1 online resource (xi, 340 pages) : : illustrations

Edition Identifiers

ISBN:9781617791888, 1617791881
DOI:10.1007/978-1-61779-188-8
oclc:ocn746342570
LC Classification:QH345
Dewey Decimal:572.8/633

Subjects

Biochemie biochemistry Biologie (algemeen) Biology (General) Chemicals and Drugs Chemie Chemistry Internet resources Laboratory manuals levenswetenschappen Life Sciences Nucleic Acids Nucleic Acids, Nucleotides, and Nucleos… Nucléotides Oligonucléotides Oligonucléotides -- Emploi en thérapeut… Oligonucleotides -- Therapeutic use Polynucléotides