Tumor angiogenesis assays: methods and protocols

by Ribatti, Domenico

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Title Statements

Full title :Tumor angiogenesis assays : methods and protocols / edited by Domenico Ribatti.

Summary

This second edition provides new and updated methods and protocols for studying tumor angiogenesis in vitro and in vivo. Chapters detail morphological aspects of tumor angiogenesis, aortic ring, ex vivo tissue culture model for anti-angiogenic drug testing, transgenic zebrafish, orthotopic models of ovarian cancer, and uncovering metabolic effects of anti-angiogenic therapy in tumors by induced metabolic bioluminescence imaging.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Tumor Angiogenesis Assays: Methods and Protocols, Second Edition is a valuable resource for all researchers interested in learning more about this important and developing field.

Edition Statements

Publication Date:2023.
Edition:Second edition

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:2023.

Notes

Includes index.

The Physical Object

Description:1 online resource (xi, 212 pages) : : illustrations (some color).

Edition Identifiers

ISBN:9781071627037, 1071627031
DOI:10.1007/978-1-0716-2703-7
oclc:on1346659067
LC Classification:RC269
Dewey Decimal:616.99/4061

Discovery and development of tumor angiogenesis assays -- The role of the stroma in tumor angiogenesis and progression: the fundamental contribution of Pietro M. Gullino -- Methods for Isolation of Tumor Associated Endothelial Cells for Surface Protein Analysis and Sorting by Flow cytometry -- Perfusion based fluorescent dye labeling to sort cancer cells based on their distance from blood vessels -- Visualizing the interactions that haping the imaging of microenvironment in cancer diseases -- Quantification of tumor and angiogenic-related markers in ovarian cancer models by a digital pathology approach -- Monitoring angiotropic extravascular migratory metastasis in vitro -- Double immunohistochemical staining on formalin-fixed paraffin-embedded tissue samples to study vascular co-option -- Development of Experimental Three-Dimensional Tumor Models to Study Glioblastoma Cancer Stem Cells and Tumor Microenvironment -- IKOSAª CAM Assay application to quantify blood vessels on chick chorioallantoic membrane (CAM) -- Application of laser speckle contrast imaging (LSCI) for the angiogenesis measurement of tumors in the chorioallantoic membrane (CAM) model -- The chick CAM as an in vivo …