Vaccinia Virus

Vaccinia Virus: Methods and Protocols

by Mercer, Jason

Type Book
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Title Statements

  • Full title :Vaccinia Virus : Methods and Protocols.

Edition Statements

  • Publication Date:2019.

Publication Statements

  • Publisher(s):Springer
  • Place of Publication:New York, NY
  • Language:eng
  • Publication Date:2019.

Notes

3.5 Plaque Selection and Purification

The Physical Object

  • Description:1 online resource (318 pages)

Edition Identifiers

  • ISBN:9781493995936, 1493995936
  • oclc:on1122910580
  • LC Classification:QR355-502

Table of Contents

Intro; Foreword; Preface; Acknowledgments; Contents; Contributors; Chapter 1: Working Safely with Vaccinia Virus: Laboratory Technique and Review of Published Cases of Accidental Laboratory Infections with Poxviruses; 1 Introduction; 2 Materials and Equipment; 3 Methods; 3.1 Laboratory and Personal Protective Equipment; 3.2 Laboratory Safety; 3.3 Occupational Smallpox Vaccination; 3.4 Future Considerations; 4 Notes; References; Chapter 2: Bioinformatics for Analysis of Poxvirus Genomes; 1 Introduction; 2 Materials; 3 Methods; 3.1 Connecting to the VBRC Web Site

3.2 Organizing Poxvirus Genome Data3.3 Viral Orthologous Clusters; 3.3.1 VOCs Use Example #1: Alignment of Poxvirus Uracil-DNA Glycosylase (UNG) Proteins; 3.3.2 VOCs Use Example #2: Display Those Ortholog Groups that Have a Protein Member Present in Myxoma Virus but Not in Rabbit Fibroma Virus; 3.3.3 VOCs Use Example #3: Find Ortholog Groups that Have Gene Present in All Poxvirus Genomes; 3.3.4 VOCs Use Example #4: Draw Genome Map; 3.3.5 VOCs Use Example #5: Create Sets of Concatenated Genes from Multiple Genomes; 3.4 The Viral Genome Organizer

3.4.1 VGO Use Example #1: Validating a Small ORF as a Real Gene (See Note 7)3.…

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