Yeast functional genomics: methods and protocols

by Devaux, Frédéric

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Title Statements

Full title :Yeast functional genomics : methods and protocols / edited by Frédéric Devaux.

Summary

This second edition volume discusses the latest techniques and protocols used in the field that were not covered in the previous edition. The chapters in this book are organized into five parts. Part One looks at transcriptomic analyses and Part Two covers DNA replication and protein/DNA interactions. Part Three discusses translation dynamics, protein complexes, and proteomics. Part Four looks at genotypic screens and phenotypic profiling, and Part Five explores in silico integration of functional genomics data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary material and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and practical, Yeast Functional Genomics: Methods and Protocols, Second Edition is a valuable resource for all researchers interested in learning more about the evolving field of yeast.

Edition Statements

Publication Date:2022
Edition:Second edition

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:2022

Notes

Previous edition: 2016.

The Physical Object

Description:xv, 474 pages : : illustrations (some color) ; : 26 cm.

Edition Identifiers

ISBN:9781071622568, 1071622560
oclc:on1308555987
LC Classification:QK623.S23
Dewey Decimal:579.563135

Single-Cell RNA-Sequencing in Yeast using the 10x Genomics Chromium Device -- Genome-Wide Profiling of Transcription Initiation with STRIPE-seq -- A Modified Crosslinking Analysis of cDNAs (CRAC) Protocol for Detecting RNA-Protein Interactions and Transcription at Single Nucleotide Resolution -- High-Resolution Deep Sequencing of Nascent Transcription in Yeast with BioGRO-seq -- Direct Sequencing of RNA and RNA Modification Identification using Nanopore -- UNAGI: Yeast Transcriptome Reconstruction and Gene Discovery using Nanopore Sequencing -- Identification of Taxonomically Restricted Transcripts from Illumina RNA Sequencing Data -- FORK-seq: Single Molecule Profiling of DNA Replication -- CUT&RUN Profiling of the Budding Yeast Epigenome -- ChIP-SICAP: A New Tool to Explore Gene-Regulatory Networks in Candida albicans and Other Yeasts -- Purification of Ribosome-Nascent-Chain Complex for Ribosome Profiling and Selective Ribosome Profiling -- Single-Step Affinity Purification (ssAP) and Mass Spectrometry of Macromolecular Complexes in the Yeast S. cerevisiae -- Composition and Dynamics of Protein Complexes Measured by Quantitative Mass Spectrometry of Affinity Purified Samples --…

Subjects

Functional genomics Genetics -- Technique Génomique fonctionnelle Levures (Botanique) -- Génétique Medical laboratory technology Molecular genetics -- Technique Saccharomyces cerevisiae -- genetics Yeast fungi -- Genetics