A practical guide to membrane protein purification
by Jagow, Gebhard von Schägger, Hermann
A Practical Guide to Membrane Protein Purification is written especially for researchers who have some familarity with separation of water-soluble proteins, but who may not be aware of the pitfalls they face with membrane proteins. This guide presents techniques in a concise form, emphasizing the aspects unique to membrane proteins. The book explains the principles of the methods, permitting researchers and students new to this area to adapt these techniques to their particular needs. The secon…
Read More ↓Title Statements
- Full title :A practical guide to membrane protein purification / edited by Gebhard von Jagow, Hermann Schagger.
Summary
A Practical Guide to Membrane Protein Purification is written especially for researchers who have some familarity with separation of water-soluble proteins, but who may not be aware of the pitfalls they face with membrane proteins. This guide presents techniques in a concise form, emphasizing the aspects unique to membrane proteins. The book explains the principles of the methods, permitting researchers and students new to this area to adapt these techniques to their particular needs. The second volume in the series, this book is an essential manual for investigations of structure and function of native membrane proteins, as well as for purification of these proteins for immunization and protein sequencing. Separation, Detection, and Characterization of Biological Macromolecules is a new series of laboratory guides. Each volume focuses on a topic of central interest to scientists and students in biomedical and biological research. Introductory chapters are followed by clear, step-by-step protocols that present principles and practice. These concise manuals are designed for optimal understanding of methods as well as for practical benchtop use.
Read More ↓Edition Statements
- Publication Date:©1994
Publication Statements
- Publisher(s):Academic Press
- Place of Publication:San Diego
- Language:eng
- Publication Date:©1994
The Physical Object
- Description:1 online resource (xvii, 166 pages) : : illustrations
Edition Identifiers
- ISBN:9780080571720, 0080571727
- oclc:ocn881853640
- LC Classification:QP552.M44
- Dewey Decimal:574.19/245
Table of Contents
Front Cover; A Practical Guide to Membrane Protein Purification; Copyright Page; Table of Contents; Contributors; Preface to the Series; Preface to the Volume; Abbreviations; PART I: STRATEGIES AND TECHNIQUES; Chapter 1. Purification Strategies for Membrane Proteins; I. INTRODUCTION; II. GENERAL GUIDE FOR RETAINING CATALYTIC ACTIVITY; III. CHOICE AND SEQUENCE OF PURIFICATION TECHNIQUES; IV. CHOICE OF PROTEIN SOURCE, DISRUPTION OF CELLS, AND PREPARATION OF ORGANELLES AND MEMBRANES; V. PROTEIN ASSAY; VI. SOLUBILIZATION AND STABILIZATION OF MEMBRANE PROTEINS; REFERENCES
Chapter 2. Chromatographic Techniques and Basic Operations in Membrane Protein PurificationI. INTRODUCTION; II. SOLUBILIZATION-PRECIPITATION; III. CONCENTRATION OF SAMPLES, EXCHANGE OF BUFFER, AND EXCHANGE OF DETERGENT; IV. CHROMATOGRAPHIC TECHNIQUES; REFERENCES; Chapter 3. Denaturing Electrophoretic Techniques; I. INTRODUCTION; II. CHOICE OF OPTIMAL SDS-PAGE SYSTEM AND GEL TYPE; III. TRICINE-SDS-PAGE; IV. COOMASSIE BLUE-SDS-PAGE AND QUANTITATIVE RECOVERY OF LARGE MEMBRANE PROTEINS AND PEPTIDES; V. ELECTROBLOTTING; REFERENCES; Chapter 4. Native Gel Electrophoresis; I. INTRODUCTION
II. NATIVE ELECTROPHORESIS: TECHNIQ…
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