A practical guide to membrane protein purification

A practical guide to membrane protein purification

by Jagow, Gebhard von Schägger, Hermann

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Title Statements

  • Full title :A practical guide to membrane protein purification / edited by Gebhard von Jagow, Hermann Schagger.

Edition Statements

  • Publication Date:©1994.

Publication Statements

  • Publisher(s):Academic Press
  • Place of Publication:San Diego
  • Language:eng
  • Publication Date:©1994.

The Physical Object

  • Description:xvii, 166 pages : : illustrations ; : 24 cm

Edition Identifiers

  • ISBN:0127255508, 9780127255507
  • oclc:ocm30110539
  • LC Classification:QP552.M44
  • Dewey Decimal:574.19/245

Table of Contents

1. Purification Strategies for Membrane Proteins / Gebhard Von Jagow, Thomas A. Link and Hermann Schagger -- 2. Chromatographic Techniques and Basic Operations in Membrane Protein Purification / Hermann Schagger -- 3. Denaturing Electrophoretic Techniques / Hermann Schagger -- 4. Native Gel Electrophoresis / Hermann Schagger -- 5. Isolation of Complex III, Subcomplexes, and Protein Subunits from Bovine Heart: Triton X-100/Hydroxylapatite / Hermann Schagger -- 6. Chromatographic Purification of F[subscript 1]F[subscript 0]-ATPase and Complex I from Bovine Heart Mitochondria: FPLC-Anion-Exchange and Dye-Ligand Chromatography / Susan K. Buchanan and John E. Walker -- 7. Isolation of Ubiquinol: Cytochrome c Oxidoreductase (Complex III) from Fungal and Plant Mitochondria: Affinity Chromatography / Martin Bodicker and Hanns Weiss -- 8. Isolation of Wolinella succinogenes Hydrogenase: Chromatofocusing / Achim Kroger, Viktor Geisler and Andrea Duchene.

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