Adipose-derived stem cells: methods and protocols

by Bunnell, Bruce A. Gimble, Jeffrey M.

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Title Statements

Full title :Adipose-derived stem cells : methods and protocols / edited by Jeffrey M. Gimble, Bruce A. Bunnell.

Summary

During the past decade, a wide range of scientific disciplines have adopted the use of adipose-derived stem/stromal cells (ASCs) as an important tool for research and discovery. In Adipose-Derived Stem Cells: Methods and Protocols, experts from the field, including members of the esteemed International Federation of Adipose Therapeutics and Science (IFATS), provide defined and established protocols in order to further codify the utilization of these powerful and accessible cells. With chapters organized around approaches spanning the discovery, pre-clinical, and clinical processes, much of the emphasis is placed on human ASC, while additional techniques involving small and large animal species are included. As a volume in the highly successful Methods in Molecular Biology"!series, the detailed contributions include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Adipose-Derived Stem Cells: Methods and Protocols serves as a vital reference text for experienced researchers as well as new students on the path to further exploring the incredible potential of ASCs

Edition Statements

Publication Date:©2011.

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:©2011.

The Physical Object

Description:xv, 473 pages : : illustrations (some color) ; : 27 cm

Edition Identifiers

ISBN:9781617379598, 161737959X, 9781617379604, 1617379603
oclc:ocn663950237
LC Classification:QH588.S83
Dewey Decimal:616.02774

Isolation and culture of rhesus adipose-derived stem cells / Christine Gagliardi and Bruce A. Bunnell -- Isolation of human adipose-derived stem cells from lipoaspirates / Gang Yu [and others] -- Isolation of murine adipose-derived stem cells / Gang Yu [and others] -- In vitro adult rat adipose tissue-derived stromal cell isolation and differentiation / Mandi J. Lopez and Nakia D. Spencer -- In vitro adult canine adipose tissue-derived stromal cell growth characteristics / Nakia D. Spencer and Mandi J. Lopez -- Adipogenic differentiation of adult equine mesenchymal stromal cells / Martin A. Vidal and Mandi J. Lopez -- Isolation and culture of porcine adipose tissue-derived somatic stem cells / Kellie J. Williams, Robert A. Godke, and Kenneth R. Bondioli -- Automated isolation and processing of adipose-derived stem and regenerative cells / Kevin C. Hicok and Marc H. Hedrick -- Methods for the purification and characterization of human adipose-derived stem cells / Stan Gronthos and Andrew C.W. Zannettino -- Characterization of human adipose-derived stem cells using flow cytometry / H. Alan Tucker and Bruce A. Bunnell -- Evaluation of cellular and humoral immune responses to allogene…

Subjects

Adipose Tissue Adipose Tissue -- cytology Adipose tissues Biowissenschaften Cell culture Cell Culture Techniques Cells, Cultured Cellules -- Culture Cellules souches Cellules souches mésenchymateuses Laboratory Manual Laboratory manuals Manuels de laboratoire Mesenchymal Stem Cells Stem cells Tissu adipeux