Alternative splicing: methods and protocols

by Mauger, Oriane Scheiffele, Peter

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Title Statements

Full title :Alternative splicing : methods and protocols / edited by Peter Scheiffele and Oriane Mauger.

Summary

This detailed volume collects commonly used and cutting-edge methods to analyze alternative splicing, a key step in gene regulation. After an introduction of the alternative splicing mechanism and its targeting for therapeutic strategies, the book continues with techniques for analyzing alternative splicing profiles in complex biological systems, visualizing and localizing alternative spliced transcripts with cellular and sub-cellular resolution, probing regulators of alternative splicing, as well as assessing the functional consequences of alternative splicing. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Alternative Splicing: Methods and Protocols serves as an ideal guide for both RNA aficionados that want to implement novel approaches in their labs and novices undertaking alternative splicing projects.

Edition Statements

Publication Date:2022.

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, N.Y
Language:eng
Publication Date:2022.

Notes

Includes index.

The Physical Object

Description:1 online resource.

Edition Identifiers

ISBN:9781071625217, 1071625217
DOI:10.1007/978-1-0716-2521-7
oclc:on1337857072
LC Classification:QH442
Dewey Decimal:660.6/5

Alternative Splicing in Human Biology and Disease -- Targeting Alternative Splicing for Therapeutic Interventions -- An Optimized Protocol for the Mapping of Cell Type-Specific Ribosome-Associated Transcript Isoforms from Small Mouse Brain Regions -- FACS-Based Neuronal Cell Type-Specific RNA Isolation and Alternative Splicing Analysis -- Quantitative Measurement of Alternatively Spliced RNA Isoform Levels -- Analysis of Splicing Regulation by Third-Generation Sequencing -- Computational Analysis of Alternative Splicing Using VAST-TOOLS and the VastDB Framework -- Identification and Quantification of Microexons Using Bulk and Single-Cell RNA-Seq Data -- Functional Annotation of Custom Transcriptomes -- Design, Labeling, and Application of Probes for RNA smFISH -- BaseScope"!Approach to Visualize Alternative Splice Variants in Tissue -- In Situ Imaging of mRNA Splicing Variants by SpliceRCA -- Two-Color Fluorescent Reporters for Analysis of Alternative Splicing -- Quantitative Detection of Protein Splice Variants by Selected Reaction Monitoring (SRM) Mass Spectrometry -- Probing the Interactions of Splicing Regulatory Small Molecules and Proteins with U1 snRNP Using NMR Spectroscop…