Amino acid analysis: methods and protocols

by Alterman, Michail A. Hunziker, Peter

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Title Statements

Full title :Amino acid analysis : methods and protocols / edited by Michail A. Alterman, Peter Hunziker.

Summary

Amino Acid Analysis (AAA) is an integral part of analytical biochemistry. In a relatively short time, the variety of AAA methods has evolved dramatically with more methods shifting to the use of mass spectrometry (MS) as a detection method. Another new aspect is miniaturization. However, most importantly, AAA in this day and age should be viewed in the context of Metabolomics as a part of Systems Biology. Amino Acid Analysis: Methods and Protocols presents a broad spectrum of all available methods allowing for readers to choose the method that most suits their particular laboratory set-up and analytical needs. In this volume, a reader can find chapters describing general as well as specific approaches to the sample preparation. A number of chapters describe specific applications of AAA in clinical chemistry as well as in food analysis, microbiology, marine biology, drug metabolism, even archeology. Separate chapters are devoted to the application of AAA for protein quantitation and chiral AAA. Written in the highly successful Methods in Molecular Biology"!series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Amino Acid Analysis: Methods and Protocols provides crucial techniques that can be applied across multiple disciplines by anyone involved in biomedical research or life sciences

Edition Statements

Publication Date:©2012.

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York
Language:eng
Publication Date:©2012.

The Physical Object

Description:xiv, 363 pages : : illustrations (some color) ; : 26 cm

Edition Identifiers

ISBN:9781617794445, 1617794449, 9781617794452, 1617794457
oclc:ocn751836108
LC Classification:QP561
Dewey Decimal:572.65

Rapid LC-MS/MS profiling of protein amino acids and metabolically related compounds for large-scale assessment of metabolic phenotypes -- Combination of an AccQ. Tag-ultra performance liquid chromatographic method with tandem mass spectrometry for the analysis of amino acids -- Isotope dilution liquid chromatography-tandem mass spectrometry for quantitative amino acid analysis -- Analysis of underivatized amino acids: Zwitterionic hydrophilic interaction chromatography combined with triple quadrupole tandem mass spectrometry -- Amino acid analysis via LC-MS method after derivatization with quaternary phosphonium -- Amino acid analysis by hydrophilic interaction chromatography coupled with isotope dilution mass spectrometry -- A universal HPLC-MS method to determine the stereochemistry of common and unusual amino acids -- Amino acid analysis by capillary electrophoresis-mass spectrometry -- New advances in amino acid profiling by capillary electrophoresis-electrospray ionization-mass spectrometry -- Optimal conditions for the direct RP-HPLC determination of underivatized amino acids with online multiple detection -- Absolute quantitation of proteins by acid hydrolysis combined with…

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