AMPK: Methods and Protocols
by Neumann, Dietbert Viollet, Benoit
This volume explores the latest methods used to study AMPK by computational, biochemical, biophysical, cellular, and ex vivo and in vivo approaches. The chapters in this book cover specific topics, such as methods to measure change in cellular energy metabolism and analyze metabolic pathways regulated by AMPK; bioinformatics tools to identify AMPK targets; knockdown of AMPK by CRISPR-Cas9; production and crystallization of full-length human AMP-activated protein kinase; cell-free assays to meas…
Read More ↓Title Statements
- Full title :AMPK : Methods and Protocols / edited by Dietbert Neumann, Benoit Viollet.
Summary
This volume explores the latest methods used to study AMPK by computational, biochemical, biophysical, cellular, and ex vivo and in vivo approaches. The chapters in this book cover specific topics, such as methods to measure change in cellular energy metabolism and analyze metabolic pathways regulated by AMPK; bioinformatics tools to identify AMPK targets; knockdown of AMPK by CRISPR-Cas9; production and crystallization of full-length human AMP-activated protein kinase; cell-free assays to measure the effects of regulatory ligands on AMPK; use of sensors of AMPK activity; AMPK protein interaction by yeast two-hybrid; the role of AMPK in inflammation and autophagy; analyzing the AMPK function in C. elegans and mammals (with special focus on skeletal muscle, blood vessels, kidneys, pancreatic islets and hypothalamus); and human?2 AMPK mutations. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and thorough, AMPK: Methods and Protocols is a valuable resource for graduate students, postdoctoral researchers, and established investigators who are interested in the richly diverse AMPK field.
Read More ↓Edition Statements
- Publication Date:2018.
Publication Statements
- Publisher(s):Humana Press, Imprint, Springer New York
- Place of Publication:New York, NY
- Language:eng
- Publication Date:2018.
The Physical Object
- Description:1 online resource (XVII, 626 pages 119 illustrations, 76 illustrations in color.) : : online resource
Edition Identifiers
- ISBN:9781493975983, 1493975986, 9781493975976, 1493975978
- DOI:10.1007/978-1-4939-7598-3
- oclc:on1034546863
- LC Classification:QD431-431.7
- Dewey Decimal:572.6
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