Germline development: methods and protocols

by Blomberg, Le Ann Chan, Wai-Yee

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Title Statements

Full title :Germline development : methods and protocols / edited by Wai-Yee Chan, Le Ann Blomberg.

Edition Statements

Publication Date:©2012.

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:©2012.

The Physical Object

Description:1 ressource en ligne (1 texte électronique (xi, 256 pages)) : : illustrations (certaines en couleur), fichiers PDF

Edition Identifiers

ISBN:1617794368, 9781617794360
oclc:on1132045120
LC Classification:QL964
Dewey Decimal:571.8/45

Isolation of fetal gonads from embryos of timed-pregnant mice for morphological and molecular studies -- Neonatal testicular gonocytes isolation and processing for immunocytochemical analysis -- Isolation of undifferentiated and early differentiating type a spermatogonia from pou5f1-GFP reporter mice -- Isolation of human male germ-line stem cells using enzymatic digestion and magnetic-activated cell sorting -- Isolation and purification of murine male germ cells -- Preparation of enriched mouse syncitia-free pachytene spermatocyte cell suspensions -- Revealing the transcriptome landscape of mouse spermatogonial cells by tiling microarray -- Biochemical characterization of a testis-predominant isoform of n-[alpha] acetyltransferase -- Identification of novel long noncoding RNA transcripts in male germ cells -- Methylation profiling using methylated DNA immunoprecipitation and tiling array hybridization -- Spermatogenesis in cryptorchidism -- In vitro culture of fetal ovaries : A model to study factors regulating early follicular development -- Microspread ovarian cell preparations for the analysis of meiotic prophase progression in oocytes with improved recovery by cytospin centri…