Angiogenesis protocols

by Pang, Jian-Min

Type Book

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Title Statements

Full title :Angiogenesis protocols / editors: Stewart G. Martin, Peter W. Hewett.

Summary

"In addition to updating important core techniques this third edition presents new chapters on assessing leukocyte involvement in angiogenesis, lymphatic cell and pericyte isolation techniques, spheroid and arterial ring based in vitro assays, and on pericyte involvement in angiogenesis. Angiogenesis Protocols, Third Edition also guides readers through new protocols on chorioallantoic membrane models, corneal pocket assays to assess angio- and lymphangiogenesis, models of muscle angiogenesis, and use of zebrafish embryos to study vascular angiogenesis and senescence. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Angiogenesis Protocols, Third Edition provides not only a practical handbook for key techniques, but also an informative and enjoyable read for all those interested, no matter how directly, in angiogenesis"--OCLC.

Edition Statements

Publication Date:2016.
Edition:Third edition

Publication Statements

Publisher(s):Humana Press
Place of Publication:New york
Language:eng
Publication Date:2016.

The Physical Object

Description:1 online resource (1 recurs en línia (450 p.) :) : : il.lustracions.

Edition Identifiers

ISBN:9781493936281, 149393628X
oclc:ocn970441711

Conté: Assessing Tumor Angiogenesis in Histological Samples / Jia-Min Pang ... -- Immunohistochemical Methods for Measuring Tissue Lymphangiogenesis -- Immunohistochemical Assessment of Leukocyte Involvement in Angiogenesis -- Isolation and Culture of Human Endothelial Cells from Micro- And Macro-Vessels -- Isolation, Identification and Culture of Human Lymphatic Endothelial Cells -- Isolation, Culture and Characterisation Of Vascular Smooth Muscle Cells -- Isolation and Transfection of Primary Culture Bovine Retinal Pericytes -- In Vitro Assays for Endothelial Cell Functions Required for Angiogenesis: Proliferation, Motility, Tubular Differentiation And Matrix Proteolysis -- Tube Forming Assays -- In Vitro Co-Culture Assays of Angiogenesis -- Spheroid-Based In Vitro Angiogenesis Model -- Stem Cell Spheroid-Based Sprout Assay in Three-Dimensional Fibrin Scaffold: A Novel In Vitro Model for the Study Of Angiogenesis -- Human Arterial Ring Angiogenesis Assay -- A Modified Aortic Ring Assay to Assess Angiogenic Potential In Vitro -- Quantitative Imaging-Based Examination of Pericyte Control of Endothelial Growth Dynamics and Angiogenesis -- Static and Dynamic Assays of Cell Adhesion …