Antigen Processing: Methods and Protocols

by Van Endert, Peter

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Title Statements

Full title :Antigen Processing : Methods and Protocols / edited by Peter van Endert.

Summary

"The second edition volume expands on the previous edition with new and updated chapters on the latest methods used for studying presentation of antigenic peptides produced in the standard processing pathways for MHC class I and II molecules. The new chapters cover topics such as biochemical and cellular approaches to study the impact of the endoplasmic reticulum aminopeptidases; techniques to monitor MHC class I synthesis and degradation; approaches to measure processing efficacy; description of different assays measuring MHC recycling; and protocols to produce MHC class II tetramers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Antigen Processing: Methods and Protocols, Second Edition is a valuable tool for both novice and expert researchers interested in studying antigen processing and venturing out further into this evolving field." - Prové de l'editor.

Edition Statements

Publication Date:2019
Edition:2nd ed. 2019

Publication Statements

Publisher(s):Imprint: Humana, Springer New York
Place of Publication:New York, NY
Language:eng
Publication Date:2019

The Physical Object

Description:1 recurs en línia (XV, 458 pàgines) : : 79 il·lustracions, 45 il·lustracions en color

Edition Identifiers

ISBN:9781493994502, 1493994506
DOI:10.1007/978-1-4939-9450-2
oclc:on1139499167

Conté: Purification of Large Cytosolic Proteases for In Vitro Assays: 20S and 26S Proteasomes -- Analysis of Proteasome Generated Antigenic Peptides by Mass Spectrometry -- Trimming of MHC Class I Ligands by ERAP Aminopeptidases -- Peptide Trimming for MHC Class I Presentation by Endoplasmic Reticulum Aminopeptidases -- Testing the Impact of Protease Inhibitors in Antigen Presentation Assays -- In Vitro Studies of MHC Class I Peptide Loading and Exchange -- Measuring Synthesis and Degradation of MHC Class I Molecules -- Biochemical Analysis of Naturally Processed Antigenic Peptides Presented by MHC Class I Molecules -- A SIINFEKL Based System to Measure MHC Class I Antigen Presentation Efficiency and Kinetics -- Purification and Identification of Naturally Presented MHC Class I and II Ligands -- Identification of MHC Ligands and Establishing MHC Class I Peptide Motifs -- Quantitating MHC Class I Ligand Production and Presentation using TCR-Like Antibodies -- Identifying Antigens Recognized by Cytolytic T Lymphocytes on Tumors -- A Flow Cytometry-Based Approach to Unravel Viral Interference with the MHC Class I Antigen Processing and Presentation Pathway -- Evaluating CD8+ T Cell R…