Basic Cell Culture Protocols

by Helgason, Cheryl D. Miller, Cindy L.

Type Book

Rate 0.0 /5

Details

Files

Reviews

Download

Title Statements

Full title :Basic Cell Culture Protocols / edited by Cheryl D. Helgason, Cindy L. Miller.

Summary

At some point in their careers, virtually every scientist and technician, as well as many medical professionals, regardless of their area of specialization have a need to utilize cell culture systems. Updating and significantly expanding upon the previous editions, Basic Cell Culture Protocols, Fourth Edition provides the novice cell culturist with sufficient information to perform the basic techniques, to ensure the health and identity of their cell lines, and to be able to isolate and culture specialized primary cell types. The intent of this extensive volume is to generate a valuable resource containing clear methodologies pertinent to current areas of investigation, rather than attempting to educate cell culturists on specific cell types or organ systems. Written in the highly successful Methods in Molecular Biology"! chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Basic Cell Culture Protocols, Fourth Edition compiles the essential techniques needed to approach this vital laboratory activity with full success

Edition Statements

Publication Date:2013.
Edition:4th ed. 2013

Publication Statements

Publisher(s):Humana Press, Imprint
Place of Publication:Totowa, NJ
Language:eng
Publication Date:2013.

Notes

Bibliographic Level Mode of Issuance: Monograph.

The Physical Object

Description:1 online resource (XIV, 550 pages 104 illustrations, 44 illustrations in color.)

Edition Identifiers

ISBN:1627031286, 9781627031288, 9781627031271, 1627031278, 9781627031295, 1627031294, 9781493956524, 1493956523
DOI:10.1007/978-1-62703-128-8.
oclc:on1109036471
LC Classification:QH573-671
Dewey Decimal:571.638

1. Detection of Mycoplasma Contaminations -- 2. Eradication of Mycoplasma Contaminations -- 3. STR DNA Typing of Human Cell Lines: Detection of Intra- and Interspecies Cross-Contamination -- 4. Classical and Molecular Cytogenetic Analysis -- 5. Fluorescent In Situ Hybridization (FISH) of DNA Probes in the Interphase and Metaphase Stages of the Cell Cycle -- 6. The Development of T Lymphocytes in Fetal Thymus Organ Culture -- 7. Generation, Isolation, and Engraftment of In Vitro-Derived Human T Cell Progenitors -- 8. In Vitro Generation of Human T Regulatory Cells: Generation, Culture, and Analysis of FOXP3-Transduced T Cells -- 9. Simultaneous Cloning and Selection of Hybridomas and Transfected Cell Lines in Semisolid Media -- 10. Isolation and Characterization of Mouse Side Population Cells -- 11. Stem Cell Identification by DyeCycle Violet Side Population Analysis -- 12. Isolation and Characterization of Cancer Stem Cells In Vitro -- 13. Ex Vivo Differentiation of Cord Blood Stem Cells into Megakaryocytes and Platelets -- 14. Generation and Characterization of Murine Alternatively Activated Macrophages -- 15. Human Long Term Culture Initiating Cell (LTC-IC) Assay -- 16. Long Ter…

Subjects

Analytical, Diagnostic and Therapeutic … Cell culture Cell culture -- Laboratory manuals Cell Culture Techniques Cellules -- Culture Cellules -- Manuels de laboratoire. -- … Clinical Laboratory Techniques Culture Techniques Cultures (Biologie) Cultures (Biology) Investigative Techniques Laboratory manuals Médecine -- Laboratoires Medical laboratory technology Methodologie methodology Methods Publication Characteristics Publication Formats tissue culture