Calorimetry

by Feig, Andrew L.

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Title Statements

Full title :Calorimetry / edited by Andrew L. Feig.

Edition Statements

Publication Date:©2016
Edition:First edition

Publication Statements

Publisher(s):Academic Press
Place of Publication:Cambridge, MA
Language:eng
Publication Date:©2016

The Physical Object

Description:1 online resource (xix, 493 pages) : : illustrations (chiefly color)

Edition Identifiers

ISBN:9780128029077, 0128029072
oclc:ocn934769600
LC Classification:QC291
Dewey Decimal:536.6

6. Additional Considerations with Metal Ions6.1. Comparison with Other Methods; 6.2. Sources of Error; 6.3. Tips; 7. Conclusion; Acknowledgments; References; Chapter Two: Assessing Coupled Protein Folding and Binding Through Temperature-Dependent Isothermal Titration Calorimetry; 1. Introduction; 2. Sample Preparation; 2.1. Plasmid Construction; 2.2. Purification of FCP1 and Rap74; 3. Special Considerations for ITC of Intrinsically Disordered Proteins; 3.1. Experimental Design; 3.2. Calorimetric Parameters; 4. Data Fitting and Analysis; 4.1. Standard Data Fitting in Origin

4.2. Data Fitting in MATLAB4.3. Global Analysis of Temperature-Dependent Data Sets; 5. Summary; Acknowledgments; References; Chapter Three: Fragment-Based Screening for Enzyme Inhibitors Using Calorimetry; 1. Introduction; 1.1. Nanocalorimeter Arrays; 1.2. Fragment-Based Lead Discovery; 2. Required Materials; 2.1. Equipment; 2.2. Reagents; 2.3. Software; 3. Instrumentation; 4. Fragment Library Selection; 4.1. Materials Needed; 4.2. Software Needed; 4.3. Protocol to Prepare of Fragment Library for Screening; 5. Calorimetric Enzyme Assay: Nanocalorimeter

5.1. Sample Considerations and Preparation5.2. Data Analys…