Case studies in cell biology

Case Studies in Cell Biology presents real world scenarios to help readers use science process and reasoning skills. The case studies require application and analyzation of concepts beyond rote memory of biological concepts. The book is based on the student learning outcomes from the American Society for Cell Biology, offering practical application for both the classroom and research laboratory.

Subchapter 4.4 -- When It Comes to the Nucleus -- Size Matters [4].

Scanning electron microscopyResults; Subchapter 4.2 -- Importin b: The Important Importin [2]; Introduction; Background; Methods; Preparation of a high-speed supernatant from Xenopus eggs; Immunodepletion of importin from cytosol; Nuclear protein import assay; Nuclear localization signal binding assay; Protein gel electrophoresis and immunoblotting; Results; Subchapter 4.3 -- A Tale of tRNA Transport [3]; Introduction; Background; Methods; In vitro translation and 35S-labeling; Injection into Xenopus frog eggs; In vitro binding assays; Gel electrophoresis and immunoblotting; Results.

Protein expression and purificationElectron microscopy; SNARE complex labeling; Results; Subchapter 3.6 -- Second Chance Chaperones: How Misfolded Proteins get Refolded [7]; Introduction; Background; Methods; Purification of Hsp104; Purification of Hsp40 and Hsp70; Preparation of yeast lysates; Turbidity assay; Refolding assays; Gel filtration; Results; References; Chapter 4 -- The Nucleus; Subchapter 4.1 -- Nuclear Pore Complex Assembly is a House of Cards [1]; Introduction; Background; Methods; Nuclear reconstitution assay; Immuno…