Cell-Based Assays Using IPSCs for Drug Development and Testing

by Mandenius, Carl-Fredrik

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Title Statements

Full title :Cell-Based Assays Using IPSCs for Drug Development and Testing

Summary

The volume presents a variety of protocols that will be useful to researchers working in the field of disease modeling, drug discovery, and the cell biology of various tissues. Chapters in this book cover topics such as requirements and preconditions for using human induced pluripotent cell lines in assay development; the culture of iPSCs and iPSC-derived cardiomyocytes by bioreactor technologies; distinctions between hepatocytes, vascular endothelial cells, cardiomyocytes, renal podocytes, and neurons; and high content assays of mitochondrial function and autophagy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cell-Based Assay using iPSCs for Drug Development and Testing: Methods and Protocols is a valuable resource for researchers working in this important and developing field of study.

Edition Statements

Publication Date:2019.

Publication Statements

Publisher(s):Springer
Place of Publication:New York, NY
Language:eng
Publication Date:2019.

Notes

3.6 Quantitative Analysis of Differentiation Efficiency by Flow Cytometry

The Physical Object

Description:1 online resource (261 pages)

Edition Identifiers

ISBN:9781493994779, 1493994778
DOI:10.1007/978-1-4939-9477-9.
oclc:on1122923329
LC Classification:RM1-950
Dewey Decimal:615

Intro; Preface; Contents; Contributors; Chapter 1: Cell-Based Assays Using Differentiated Human Induced Pluripotent Cells; 1 Creation of a Cell Bank with Patient-Specific Induced Pluripotent Cell Lines; 2 Use of Induced Pluripotent Stem Cells in Drug Development in the Pharmaceutical Industry; 3 Differentiation of iPSCs for Assay Development; 4 Development of Protocols for iPSC-Based Assays; 4.1 Assays Related to Liquid Chromatography Combined with Tandem Mass Spectrometry (LC-MS/MS); 4.2 Assays Related to Imaging; 4.3 Assays Related to Microfluidics

4.4 Assays Related to Electrical and Fluorometric Procedures4.5 Screening Assays; References; Part I: Differentiation of iPC to Target Cells for Assays; Chapter 2: Monolayer Generation of Vascular Endothelial Cells from Human Pluripotent Stem Cells; 1 Introduction; 2 Materials; 3 Methods; 3.1 Endothelial Differentiation Method; 4 Notes; References; Chapter 3: Generating Human iPSC-Derived Astrocytes with Chemically Defined Medium for In Vitro Disease Modeling; 1 Introduction; 2 Materials; 2.1 Cell Culture Reagents; 2.2 Preparation of Stock Solutions

2.3 Laminin Coating of Cell Culture Vessels2.4 Media; 2.5 qRT-PCR reagents; 2.6 Immu…