Chemokines

by Handel, Tracy M.

Type Book

Rate 0.0 /5

Details

Files

Reviews

Download

Title Statements

Full title :Chemokines / edited by Tracy M. Handel.

Summary

Chemokines, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in chemokines, and includes sections on such topics as chemokine detection using receptors, tracking cellular responses to chemokines, recognition of GAG-bound chemokines, and the production of chemokine receptor complexes for structural and biophysical studies. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in chemokines Contains sections on such topics as chemokine detection using receptors, tracking cellular responses to chemokine, recognition of GAG-bound chemokines, and the production of chemokine receptor complexes for structural and biophysical studies.

Edition Statements

Publication Date:2016.

Publication Statements

Publisher(s):Academic Press is an imprint of Elsevier
Place of Publication:Cambridge, MA
Language:eng
Publication Date:2016.

The Physical Object

Description:1 online resource.

Edition Identifiers

ISBN:9780128021958, 0128021950
oclc:ocn942747929
LC Classification:QR185.8.C45
Dewey Decimal:616.07/9

Front Cover; Chemokines; Copyright; Contents; Contributors; Preface; Chapter One: Chemokine Detection Using Receptors Immobilized on an SPR Sensor Surface; 1. Surface Plasmon Resonance; 2. Chemokine Receptors: Members of the GPCR Family; 3. Chemokine Receptor Immobilization on the Sensor Chip; 4. Viral Particles as Chemokine Receptors Carriers in SPR; 4.1. Materials; 4.2. Method I: Generation of Retroviral Particles; 4.3. Method II: Generation of LVPs; 4.4. Method III: Titration of Viral Proteins; 4.5. Method IV: Determination of Chemokine Receptor Levels on the VP.

4.6. Method V: Quantitation of Receptor Number on VPs4.7. Method VI: Attachment of VPs to Biosensor Surfaces; 5. SPR-Based Applications for Chemokine Receptors; 6. Conclusions; Acknowledgments; References; Chapter Two: Study of Chemotaxis and Cell-Cell Interactions in Cancer with Microfluidic Devices; 1. Introduction; 2. Methods; 2.1. Chemotaxis in Microfluidic Chemotaxis Chamber; 2.1.1. Making Microfluidic Chemotaxis Chamber; 2.1.2. Chemotaxis Assay for Differentiated HL-60 Cells; 2.2. Gradient Switching in Microfluidic Device; 2.2.1. Making a Gradient Switching Microfluidic Device.

2.2.2. Chemotaxis Assay for Diffe…

Subjects

Biochemische Analyse Chemokine Chemokines Chemokines -- Physiological effect Chimiokines Chimiokines -- Effets physiologiques HEALTH & FITNESS / Diseases / General Internet resources MEDICAL -- Clinical Medicine MEDICAL -- Diseases MEDICAL -- Evidence-Based Medicine MEDICAL -- Internal Medicine Untersuchungsmethode