Chromosomal Mutagenesis

Chromosomal Mutagenesis

by Pruett-Miller, Shondra

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This new edition explores current and emerging mutagenesis methods focusing specifically on mammalian systems and commonly used model organisms through comprehensive coverage and detailed protocols. Since the first edition, major advances and discoveries have made chromosomal mutagenesis a widely used technique and one that is available to any molecular biology laboratory, and this collection provides detailed protocols, case-studies, and reviews from thought-leaders in the field. Written in th…

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Title Statements

  • Full title :Chromosomal Mutagenesis / edited by Shondra M. Pruett-Miller.

Summary

This new edition explores current and emerging mutagenesis methods focusing specifically on mammalian systems and commonly used model organisms through comprehensive coverage and detailed protocols. Since the first edition, major advances and discoveries have made chromosomal mutagenesis a widely used technique and one that is available to any molecular biology laboratory, and this collection provides detailed protocols, case-studies, and reviews from thought-leaders in the field. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

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Edition Statements

  • Publication Date:2015.
  • Edition:2. ed

Publication Statements

  • Publisher(s):Humana Press
  • Place of Publication:New York
  • Language:eng
  • Publication Date:2015.

The Physical Object

  • Description:1 online resource (online resource (xiv, 333 s.)) : : illustrations

Edition Identifiers

  • ISBN:9781493918621, 1493918621
  • oclc:ocn941095970

Table of Contents

1 Genome Editing by Targeted Chromosomal Mutagenesis / Dana Carroll -- 2 piggyBac Transposon-Based Insertional Mutagenesis in Mouse Haploid Embryonic Stem Cells / Stephen J. Pettitt, E-Pien Tan, and Kosuke Yusa -- 3 Using Phage Integrases in a Site-Specific Dual Integrase Cassette Exchange Strategy / Jonathan M. Geisinger and Michele P. Calos -- 4 Therapeutic Genome Mutagenesis Using Synthetic Donor DNA and Triplex-Forming Molecules / Faisal Reza and Peter M. Glazer -- 5 Genome Engineering Using Adeno-Associated Virus (AAV) / Rob Howes and Christine Schofield -- 6 Engineering of Customized Meganucleases via In Vitro Compartmentalization and In Cellulo Optimization / Ryo Takeuchi, Michael Choi, and Barry L. Stoddard -- 7 Efficient Design and Assembly of Custom TALENs Using the Golden Gate Platform / Tomas Cermak, Colby G. Starker, and Daniel F. Voytas -- 8 Ligation-Independent Cloning (LIC) Assembly of TALEN Genes / Jonathan L. Schmid-Burgk, Tobias Schmidt, and Veit Hornung -- 9 Assembly and Characterization of megaTALs for Hyperspecific Genome Engineering Applications / Sandrine Boissel and Andrew M. Scharenberg -- 10 Genome Engineering Using CRISPR-Cas9 System / Le Cong and Feng …

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Subjects

Chromosomes -- Recherche Chromosomes -- Research Mutagenèse -- Méthodologie Mutagenesis -- Methodology

Writers

Pruett-Miller, Shondra

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