Citrus Tristeza Virus: Methods and Protocols
This book provides methods and clear protocols for the various technologies available to detect, characterize, and study Citrus tristeza virus (CTV), a member of the genus Closterovirus, family Closteroviridae. Thanks to the highly sensitive and specific diagnostic procedures developed, knowledge of the molecular characteristics, expression strategies, genetic variability, and epidemiology of the virus has improved significantly, as this volume reflects. Written for the highly successful Method…
Read More ↓Title Statements
- Full title :Citrus Tristeza Virus : Methods and Protocols.
Summary
This book provides methods and clear protocols for the various technologies available to detect, characterize, and study Citrus tristeza virus (CTV), a member of the genus Closterovirus, family Closteroviridae. Thanks to the highly sensitive and specific diagnostic procedures developed, knowledge of the molecular characteristics, expression strategies, genetic variability, and epidemiology of the virus has improved significantly, as this volume reflects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Citrus Tristeza Virus: Methods and Protocols serves as an ideal guide for plant pathologists, plant virologists, molecular biologists, and graduate students interested in performing qualitative and quantitative tests as well as recently-developed diagnostic methods in order to find solutions to improve the management of this disease.
Read More ↓Edition Statements
- Publication Date:2019.
Publication Statements
- Publisher(s):Springer
- Place of Publication:New York, NY
- Language:eng
- Publication Date:2019.
Notes
3.3.3 Real-Time PCR: Assay B
The Physical Object
- Description:1 online resource (252 pages)
Edition Identifiers
- ISBN:9781493995585, 1493995588
- DOI:10.1007/978-1-4939-9558-5.
- oclc:on1122912785
- LC Classification:QH301-705
- Dewey Decimal:571.92
Table of Contents
Intro; Preface; Contents; Contributors; Chapter 1: A Short Note on Reflections and Publications on Citrus tristeza virus (CTV) Methodologies; References; Selected Additional Personal Publication List; Reviews and Chapters; Chapter 2: A Brief Historical Account of the Family Closteroviridae; References; Chapter 3: Phenotyping Biological Properties of CTV Isolates; 1 Introduction; 2 Materials; 2.1 Basic Facilities; 2.2 Plant Materials; 2.3 Virus Isolates; 2.4 Grafting and Budding Inoculation; 2.5 Mechanical Transmission; 3 Methods; 3.1 Seedling Preparation; 3.2 Grafting and Budding Inoculation
3.3 Leaf-Piece Graft3.4 Leaf Grafting; 3.5 Mechanical Transmission; 3.6 Testing Cross-Protective Isolates; 3.7 Symptom Assessment; 3.8 Termination; 4 Notes; References; Chapter 4: CTV Vectors and Interactions with the Virus and Host Plants; 1 Introduction; 2 Materials; 2.1 Aphid Surveys; 2.2 Aphid Rearing; 2.3 Host Plants for CTV Vector Transmission; 2.4 Detection of CTV by DASI-ELISA; 2.5 Immunocapture (IC) for Real-Time and Conventional PCR Detection of CTV in Plants; 2.6 cDNA Synthesis; 2.7 Conventional PCR; 2.8 Real-Time PCR; 2.9 Extraction of Total RNA from Aphids
2.10 One-Step Real-Tim…
Subjects
Writers
More from this Series
No reviews yet. Be the first to rate this work.
Related Works
more →
The phytopathogen: evolution and adaptation
Molecular plant pathology
Molecular plant pathology
Molecular plant pathology
Advances in hemp research
Plant Defence: Biological Control
Rate & Review