Hairy root cultures based applications: methods and protocols

by Mehrotra, Shakti Mishra, Sonal Srivastava, Vikas

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Title Statements

Full title :Hairy root cultures based applications : methods and protocols / Vikas Srivastava, Shakti Mehrotra, Sonal Mishra, editors.

Summary

The book discusses the various methods and protocols available in hairy root culture-based research. The utilization of Agrobacterium mediated genetic transformation and establishment of hairy root cultures has paved the way for large-scale secondary metabolite production in medicinal plants. Presenting recent research and offering insights from eminent research groups, the book covers a range of topics related to hairy root-based applications, including (i) establishment of hairy roots and native production of SM (ii) yield enhancement strategies for increased SM production, like elicitation (iii) hairy roots as a tool for value-added applications such as plant-microbe interaction, characterization of plant genes and root biology studies. As such it is an informative guide and experimental manual for researchers in diverse fields of plant biology.

Edition Statements

Publication Date:©2020

Publication Statements

Publisher(s):Springer
Place of Publication:Singapore
Language:eng
Publication Date:©2020

The Physical Object

Description:1 online resource (237 pages).

Edition Identifiers

ISBN:9789811540554, 9811540551
DOI:10.1007/978-981-15-4055-4
oclc:on1231210494
LC Classification:R856-R857
Dewey Decimal:575.5/4

Intro -- Preface -- Contents -- Editors and Contributors -- Chapter 1: Hairy Roots Biotechnology Unzipped: A Journey of Reality and Promises -- 1.1 Introduction -- 1.2 Contribution of HRCs for Elemental Research -- 1.3 Contribution of HRCs for Value Added Research -- 1.4 Conclusion and Way Forward -- References -- Chapter 2: Hairy Roots as a Source of Tropane Alkaloids -- 2.1 Introduction -- 2.2 Some Protocols to Enhance Production of Tropane Alkaloids in the Hairy Root Cultures of Solanaceae Family Members -- 2.2.1 Datura stramonium -- 2.2.2 Atropa belladonna -- 2.2.3 Duboisia leichhardtii

2.3 Conclusions -- References -- Chapter 3: Induction, Metabolite Analysis, and Transgenesis of Hairy Roots from Coleus forskohlii -- 3.1 Introduction -- 3.2 Protocol 1: Induction of Hairy Roots in Coleus forskohlii -- 3.3 Protocol 2: Microscopic Detection of Metabolites in Hairy Roots -- 3.4 Protocol 3: Detection of Key Metabolites in Roots and Hairy Root of C. forskohlii -- 3.5 Protocol 4: Transgene Expression in Hairy Roots of C. forskohlii -- References -- Chapter 4: Establishment of Hairy Roots of Endangered Himalayan Plant Swertia chirata: A Sustainable Alternative to Extraction...

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Subjects

Bacteriology Bacteriology (non-medical) Biology -- Technique Biology, life sciences biomedical engineering Ecological science, the Biosphere MEDICAL -- Allied Health Services Microbial ecology Plant Diseases Plant pathology & diseases Plant Roots Racines (Botanique) root (plant material) Roots (Botany) SCIENCE -- Life Sciences