Pathogenic Yersinia: Methods and Protocols

by Lawrenz, Matthew B. Vadyvaloo, Viveka

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Title Statements

Full title :Pathogenic Yersinia : Methods and Protocols / edited by Viveka Vadyvaloo, Matthew B. Lawrenz.

Summary

This volume delivers a compendium of detailed protocols to the research community in order to aid in the investigation and discovery of Yersinia virulence mechanisms using important in vivo and in vitro infection models, which have led to major advances in the field and in our understanding of Yersinia pathogen-host interactions. Beginning with a section on mouse models, the book continues with chapters covering the monitoring of bacteria during infection, invertebrate models, as well as Yersinia interaction with immune cells and immune signaling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Pathogenic Yersinia: Methods and Protocols provides a single source for researchers seeking to better understand these pathogens and the diseases they produce in humans.

Edition Statements

Publication Date:2019.
Edition:1st ed. 2019

Publication Statements

Publisher(s):Humana, Imprint, Springer New York
Place of Publication:New York, NY
Language:eng
Publication Date:2019.

The Physical Object

Description:1 online resource (XI, 259 pages 46 illustrations, 40 illustrations in color.) : : online resource

Edition Identifiers

ISBN:9781493995417, 1493995413
DOI:10.1007/978-1-4939-9541-7
oclc:on1107538178
LC Classification:QR74.8-99.5
Dewey Decimal:579.3

An intradermal model for Yersinia pestis inoculation -- Intranasal Inoculation of Mice with Yersinia pestis and Processing of Pulmonary Tissue for Analysis -- Standardized method for aerosol challenge of rodents with Yersinia pestis for modeling primary pneumonic plague -- Mouse Models of Yersiniosis -- Intravital Confocal Microscopy of Dermal Innate Immune Responses to Flea-transmitted Yersinia pestis -- Laser scanning microscopy of Yersinia pestis infected tissues -- Real-time monitoring of Yersinia pestis promoter activity by bioluminescence imaging -- Discovering Yersinia-Host Interactions by Tissue Dual RNA-seq -- Detection of cells translocated with Yersinia Yops in infected tissues using [beta]-lactamase fusions -- Monitoring of neutrophil recruitment to mice lungs during pneumonic plague -- Co-infection assay to determine Yersinia pestis competitive fitness in fleas -- Drosophila as a model for understanding the insect host of Yersinia pestis -- Intracellular assays to monitor survival and growth of Yersinia pestis within macrophages -- Human neutrophil isolation and degranulation responses to Yersinia pestis infection -- Measurement of Yersinia translocon pore formation i…