CRISPR: methods and protocols

by Charpentier, Emmanuelle Fineran, Peter C. Lundgren, Magnus

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Title Statements

Full title :CRISPR : methods and protocols / edited by Magnus Lundgren, Emmanuelle Charpentier, Peter C. Fineran.

Summary

This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels. OCLCbrA0 CRISPR: Methods and Protocols OCLCbrA0 guides readers through techniques that have been developed specifically for the analysis of CRISPR-Cas and techniques adapted from OCLCbrA0 standard protocols of DNA, RNA and protein biology. OCLCbrA0 Written in the highly successful OCLCbrA0 Methods in Molecular Biology OCLCbrA0 series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. OCLCbrA0 Authoritative and cutting-edge, OCLCbrA0 CRISPR: Methods and Protocols OCLCbrA0 provides a broad list of tools and techniques to study the interdisciplinary aspects of the prokaryotic CRISPR-Cas defense systems.

Edition Statements

Publication Date:2015.

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:2015.

The Physical Object

Description:1 online resource (xiv, 366 pages) : : illustrations (some color)

Edition Identifiers

ISBN:9781493926879, 149392687X, 1493926861, 9781493926862
DOI:10.1007/978-1-4939-2687-9
oclc:ocn909886271
LC Classification:QH450
Dewey Decimal:572.8

Investigating CRISPR RNA Biogenesis and Function using RNA-seq -- In vitro Co-reconstitution of Cas Protein Complexes -- Analysis of CRISPR pre-crRNA Cleavage -- Annotation and Classification of CRISPR-Cas Systems -- Computational Detection of CRISPR/crRNA Targets -- High-throughput CRISPR typing of Mycobacterium tuberculosis Complex and Salmonella enterica Serotype Typhimurium -- Spacer-based Macroarrays for CRISPR Genotyping -- Analysis of crRNA using Liquid Chromatography Electrospray Ionisation Mass Spectrometry (LC ESI MS) -- Rapid Multiplex Creation of Escherichia coli Strains Capable of Interfering with Phage Infection through CRISPR -- Exploring CRISPR Interference by Transformation with Plasmid Mixtures: Identification Of Target Interference Motifs in Escherichia coli -- Electrophoretic Mobility Shift Assay of DNA and CRISPR-Cas Ribonucleoprotein Complexes -- Expression and Purification of the CMR (Type III-B) Complex in Sulfolobus solfataricus -- Procedures for Generating CRISPR Mutants With Novel Spacers Acquired from Viruses Or Plasmids -- Archaeal Viruses of the Sulfolobales: Isolation, Infection, and CRISPR Spacer Acquisition -- Using the CRISPR-Cas system to Positiv…

Subjects

Bacterial Genetics Biomedicine biomedische wetenschappen CRISPR (Genetics) CRISPR-Associated Proteins -- analysis Gene Expression Gene Expression Regulation Geneeskunde (algemeen) Genetic regulation Génétique bactérienne genexpressie Laboratory Manual Laboratory manuals Manuels de laboratoire Medical genetics Medicine (General) Régulation génétique