Cytochrome P450 Protocols

by Phillips, Ian R.

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Title Statements

Full title :Cytochrome P450 Protocols / edited by Ian R. Phillips, Elizabeth A. Shephard, Paul R. Ortiz de Montellano.

Summary

Cytochromes P450 (CYPs) comprise a large superfamily of proteins that are of central importance in the detoxification or activation of a tremendous number of natural and synthetic hydrophobic xenobiotics, including many therapeutic drugs, chemical carcinogens and environmental pollutants. CYPs are important in mediating interactions between an organism and its chemical environment and in the regulation of physiological processes. Cytochrome P450 Protocols, Third Edition focuses on high-throughput methods for the simultaneous analysis of multiple CYPs, substrates or ligands. Although the emphasis is on CYPs of mammalian origin, it reflects an increasing interest in CYPs of bacterial species. Also included are chapters on cytochrome P450 reductase (the redox partner of CYPs) and the flavin-containing monooxygenases (FMOs), and metabolomic and lipidomic approaches for identification of endogenous substrates of CYPs ('de-orphanizing' CYP substrates). Written in the successful Methods in Molecular Biology"!series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Cytochrome P450 Protocols, Third Edition provides a wide range of techniques accessible to researchers in fields as diverse as biochemistry, molecular biology, pharmacology, toxicology, environmental biology and genetics

Edition Statements

Publication Date:2013.
Edition:3rd ed. 2013

Publication Statements

Publisher(s):Humana Press, Imprint
Place of Publication:Totowa, NJ
Language:eng
Publication Date:2013.

Notes

Bibliographic Level Mode of Issuance: Monograph.

The Physical Object

Description:1 online resource (XII, 319 pages 53 illustrations, 31 illustrations in color.)

Edition Identifiers

ISBN:1627033211, 9781627033213, 9781627033220, 162703322X, 9781627033206, 1627033203, 9781493962693, 1493962698
DOI:10.1007/978-1-62703-321-3.
oclc:on1108975746
LC Classification:QD431-431.7
Dewey Decimal:572.7

Bioluminescent Assays for Cytochrome P450 Enzymes. In Vitro Drug-Drug Interaction Assay -- High-Throughput Mass Spectrometric Cytochrome P450 Inhibition Screening -- The Synthesis, Characterization and Application of 13C-Methyl Isocyanide as an NMR Probe of Heme Protein Active Sites -- High-Throughput Fluorescence Assay for Cytochrome P450 Mechanism-Based Inactivators -- Identification of Endogenous Substrates of Orphan Cytochrome P450 Enzymes Through the Use of Untargeted Metabolomics Approaches -- Genetic and Mass Spectrometric Tools for Elucidating the Physiological Function(s) of Cytochrome P450 Enzymes from Mycobacterium Tuberculosis -- An Escherichia coli Expression-Based Approach for Porphyrin Substitution in Heme Proteins -- Expression in Escherichia coli of a Cytochrome P450 Enzyme with a Cobalt Protoporphyrin IX Prosthetic Group -- Nanodiscs in the Studies of Membrane-Bound Cytochrome P450 Enzymes -- Rapid LC-MS Drug Metabolite Profiling Using Bioreactor Particles -- Fluorescence-Based Screening of cytochrome P450 activities in Intact Cells -- Screening for Cytochrome P450 Reactivity with a Reporter Enzyme -- High-Throughput Fluorescence Assay of Cytochrome P450 3A4 -- T…

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