DNA Repair: Methods and Protocols

by Balakrishnan, Lata

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Title Statements

Full title :DNA Repair : Methods and Protocols.

Summary

The volume outlines techniques used to study the primary mechanisms of important DNA repair pathways. The chapters in this book are organized into five sections, each discussing a specific aspect of repair biology. Part I reviews the role of post-translational modifications of HDR, and DNA damage caused by defective telomere replication. Part II describes methods to detect and measure DNA breaks and proteins involved in DNA damage response. Part III talks about methods designed to measure DNA repair efficiency and characterize factors involved in the repair process. Part IV discusses various in vitro assays used to purify recombinant proteins that are used to study DNA repair enzyme mechanisms. Lastly, Part V focuses on methods to study the damage arising during DNA replication. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, DNA Repair: Methods and Protocols is an essential resource for novice and experienced researchers, and provides easily adaptable techniques to help them advance in their specific protein or repair pathway of interest.

Edition Statements

Publication Date:2019.

Publication Statements

Publisher(s):Springer
Place of Publication:New York, NY
Language:eng
Publication Date:2019.

Notes

3.1 Construction of TNR Oligonucleotide Substrates Containing a Synthesized DNA Base Lesion

The Physical Object

Description:1 online resource (347 pages)

Edition Identifiers

ISBN:9781493995004, 1493995006
DOI:10.1007/978-1-4939-9500-4.
oclc:on1122916698
LC Classification:RB155-155.8
Dewey Decimal:611.01816

Intro; Preface; Contents; Contributors; Part I: Reviews; Chapter 1: Homologous Recombination-Mediated DNA Repair and Implications for Clinical Treatment of Repair Defective Cancers; Abbreviations; 1 Introduction; 2 Homologous Recombination Mediated Repair; 2.1 Ionizing Radiation Induced DNA Strand Breaks; 2.2 DNA Interstrand Cross-Link Induced Damage; 3 RAD51 Paralogs Promote HR-Mediated DNA Double-Strand Break Repair; 4 Mechanisms of Ubiquitination; 5 Ubiquitin Signaling Regulates HR-Mediated DNA Repair; 6 Clinical Phenotypes of HR Defective Cancers; 7 Concluding Remarks; References

Chapter 2: Resolving Roadblocks to Telomere Replication1 Telomeric DNA; 2 Mammalian DNA Replication; 3 Challenges to DNA Replication; 3.1 Replication Stress Response; 4 Challenges to Telomeric DNA Replication; 4.1 Structure; 4.1.1 Shelterin; 4.1.2 Repetitive G-Rich DNA; 4.1.3 T-Loop; 4.2 TERRA R-Loops; 4.3 Telomeric Damage and Repair Following Replication Stress; 5 Alternative Lengthening of Telomeres; 5.1 Replication Stress Associated with the Alternative Lengthening of Telomeres; 5.2 Specific Sources of Replication Stress at ALT Telomeres; 5.2.1 Distinct Telomere Structure and Sequence

5.2.2 Chang…