Downstream processing methods
Biotechnologists review both conventional and novel isolation techniques used in industrial applications for the downstream processing of protein molecules. The techniques include primary and secondary separations during the isolation of biomolecules, as well as unique laboratory-scale research methods from academia with a potential for scale-up. Also here are various strands of the downstream biological process essential for successful product license application, including both the validation…
Read More ↓Title Statements
- Full title :Downstream processing methods / edited by Mohamed A. Desai.
Summary
Biotechnologists review both conventional and novel isolation techniques used in industrial applications for the downstream processing of protein molecules. The techniques include primary and secondary separations during the isolation of biomolecules, as well as unique laboratory-scale research methods from academia with a potential for scale-up. Also here are various strands of the downstream biological process essential for successful product license application, including both the validation of stages, and the design and validation of viral clearance stages during the purification process. The information would find most interest in the biopharmaceutical and biotechnology industries. Annotation copyrighted by Book News, Inc., Portland, OR.
Read More ↓Edition Statements
- Publication Date:©2000.
Publication Statements
- Publisher(s):Humana Press
- Place of Publication:Totowa, N.J
- Language:eng
- Publication Date:©2000.
The Physical Object
- Description:ix, 229 pages : : illustrations ; : 24 cm.
Edition Identifiers
- ISBN:0896035646, 9780896035645
- oclc:ocm41285054
- LC Classification:TP248.25.S47
- Dewey Decimal:660/.2842
Table of Contents
Downstream processing in the biotechnology industry: an overview / Manohar Kalyanpur -- Microbial cell disruption by high-pressure homogenization / Anton P.J. Middelberg -- Protein concentration and buffer exchange using ultrafiltration / Andrew L. Zydney and Ralf Kuriyel -- High-performance tangential flow filtration for protein separations / Andrew L. Zydney and Ralf Kuriyel -- Large-scale recovery of protein inclusion bodies by continuous centrifugation / Anton P.J. Middelberg -- Application of density gradient ultracentrifugation using zonal rotors in the large-scale purification of biomolecules / Mohamed A. Desai and Sandra P. Merino.
(Continued) Application of chromatography in the downstream processing of biomolecules / Mohamed A. Desai ... [et al.] -- Affinity ligand technology: optimal protein separations for downstream processing / Ken Jones and Dev Baines -- Immunoaffinity adsorption in the large-scale isolation of biomolecules / Mohamed A. Desai -- Expanded bed adsorption in the purification of biomolecules / Allan Lihme ... [et al.] -- Application of HPLC in the purification of biomolecules / Paul Bradley and Mohamed A. Desai -- Countercurrent chromatography of prote…
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