Drosophila: methods and protocols

by Dahmann, Christian

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Title Statements

Full title :Drosophila : methods and protocols / edited by Christian Dahmann.

Summary

This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and cell lineage tracing, protein inhibition and tagging, optogenetic and optochemical control of tissue mechanics, AFM measurements, sample preparation for electron microscopy, live imaging of different tissues and organs, and quantitative image analysis. Written in the format of the highly successful Methods in Molecular Biology series, chapters include an introduction to their topic, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls Authoritative and cutting-edge, Drosophila: Methods and Protocols, Third Edition serves as a useful and practical guide to new researchers and experts using Drosophila as a model system. Chapter 7 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Edition Statements

Publication Date:2022.
Edition:Third edition

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:2022.

Notes

Includes index.

The Physical Object

Description:1 online resource (xi, 424 pages) : : illustrations (some color).

Edition Identifiers

ISBN:9781071625415, 1071625411
DOI:10.1007/978-1-0716-2541-5
oclc:on1341654858
LC Classification:QL537.D76
Dewey Decimal:595.77/4

Using FlyBase, a database of Drosophila genes and genetics -- The Q-System: A versatile repressible binary expression system -- Resources and methods for the analysis of microRNA function in Drosophila -- Analysis of single-cell transcriptome data in Drosophila -- Prime editing for precise genome engineering in Drosophila -- CRISPR/Cas9 mediated precise and efficient genome editing in Drosophila -- Tissue-specific CRISPR-Cas9 screening in Drosophila -- CRISPR-based transcriptional activation in Drosophila -- Tracing and manipulating Drosophila cell lineages based on CRISPR: CaSSA and CLADES -- Studying protein function using nanobodies and other protein binders in Drosophila -- Anchor Away: A system for fast inhibition of proteins in Drosophila -- Tagging Drosophila proteins with genetically-encoded fluorophores -- Optogenetic methods to control tissue mechanics in Drosophila -- Optochemical control of cell contractility in Drosophila embryos -- Stiffness measurement of Drosophila egg chambers by atomic force microscopy -- Cultivation and live imaging of Drosophila imaginal discs -- Sample preparation and imaging of the pupal Drosophila abdominal epidermis -- FlyClear: A tissue cl…

Subjects

Animal models in research Animal models in research -- Laboratory… Drosophila Drosophila -- Laboratory manuals Drosophila melanogaster Drosophila melanogaster / Laboratory Ma… Laboratory manuals Molecular biology -- Laboratory manuals… Molecular biology -- Laboratory manuals… Molecular biology -- Research Molecular biology -- Technique