Experimental cholestasis research

by Vinken, Mathieu

Type Book

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Title Statements

Full title :Experimental cholestasis research / edited by Mathieu Vinken.

Summary

This detailed book provides a state-of-the-art compilation of protocols relevant for the experimental study of cholestasis, each written by internationally recognized research teams that have built up long-standing expertise regarding one or more aspects of experimental cholestasis research. The first half of the collection examines methods targeted towards studying established and new mechanisms of cholestasis, while the second half explores experimental models and tools to investigate mechanisms of cholestasis and/or to predict cholestatic properties of drugs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Experimental Cholestasis Research serves as an ideal guide for basic and applied researchers pursuing this vital area of research in pharmacology and toxicology.

Edition Statements

Publication Date:2019

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:2019

The Physical Object

Description:1 online resource (xiii, 399 pages) : : 74 illustrations, 60 illustrations in color

Edition Identifiers

ISBN:9781493994205, 1493994204
DOI:10.1007/978-1-4939-9420-5
oclc:on1111181170
LC Classification:RC854.C45
Dewey Decimal:616.3/6

Mechanisms of Drug-Induced Cholestasis -- LC-MS/MS Analysis of Bile Acids in In Vitro Samples -- Pipe-3D: A Pipeline Based on Immunofluorescence, 3D Confocal Imaging, Reconstructions, and Morphometry for Biliary Network Analysis in Cholestasis -- Vesicle-Based and Hepatocyte-Based Assays for Identification of Drug Candidates Inhibiting BSEP Function -- The C-DILI™ Assay: An Integrated In Vitro Approach to Predict Cholestatic Hepatotoxicity -- In Vivo Measurement of Hepatic Drug Transporter Inhibition with Radiolabeled Bile Acids -- Measuring the Impact of Bile Acids on the Membrane Order of Primary Hepatocytes and Isolated Mitochondria by Fluorescence Imaging and Spectroscopy -- The Role and Study of Mitochondrial Impairment and Oxidative Stress in Cholestasis -- Measuring Apoptosis and Necrosis in Cholestatic Liver Injury -- Necrosome Formation and Necroptosis in Experimental Cholestasis -- Proteomics in Primary Biliary Cholangitis -- Transcriptomics Analysis of Cholestatic Compounds In Vitro -- Systems Microscopy of Stress Response Pathways in Cholestasis Research -- Mouse Models for Diseases in the Cholangiocyte Lineage -- Common Bile Duct Ligation as Model for Secondary Biliar…

Subjects

Cholestase Cholestase -- Manuels de laboratoire Cholestasis Cholestasis -- Laboratory manuals Disease Models, Animal Diseases -- Animal models Drug-Related Side Effects and Adverse R… Drugs -- Side effects Hepatology Laboratory Manual Laboratory manuals Maladies -- Modèles animaux Manuels de laboratoire MEDICAL -- Gastroenterology MEDICAL -- Pharmacology Médicaments -- Effets secondaires pharmacology