Frontiers in protein structure, function, and dynamics

by Singh, Dev Bukhsh Tripathi, Timir

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Title Statements

Full title :Frontiers in protein structure, function, and dynamics / Dev Bukhsh Singh, Timir Tripathi, editors.

Edition Statements

Publication Date:2020.

Publication Statements

Publisher(s):Springer
Place of Publication:Singapore
Language:eng
Publication Date:2020.

The Physical Object

Description:1 online resource

Edition Identifiers

ISBN:9789811555305, 9811555303
DOI:10.1007/978-981-15-5530-5
oclc:on1197757541
LC Classification:QP551
Dewey Decimal:572/.6

Intro -- Preface -- Contents -- About the Editors -- 1: Protein Purification, Estimation, Storage, and Effect on Structure-Function-Dynamics -- 1.1 Introduction -- 1.2 Protein Purification -- 1.2.1 Protein Precipitation/Salting Out -- 1.2.2 Buffer Exchange/Dialysis -- 1.2.3 Immuno-Affinity Chromatography -- 1.2.4 Affinity Chromatography -- 1.2.5 Ion-Exchange Chromatography -- 1.2.6 Size-Exclusion or Gel Filtration Chromatography (SEC/GFC) -- 1.2.7 High-Pressure/Performance Liquid Chromatography (HPLC) -- 1.2.8 Hydrophobic Interaction Chromatography -- 1.3 Resolving and Display of Protein

1.6.3 Cycles of Freeze-Thaw -- 1.6.4 Organic Solvents and pH -- 1.6.5 Proteases/Peptidases -- 1.6.6 Protein Concentration -- 1.6.7 Reducing Agents -- 1.6.8 Salt Conditions and Urea -- 1.6.9 Temperature -- 1.7 Factors Affecting the Stability of Proteins -- 1.8 Effect on the Dynamics of Protein -- 1.9 Conclusions -- References -- 2: Experimental and Computational Methods to Determine Protein Structure and Stability -- 2.1 Protein Purification Techniques -- 2.1.1 General Aspects of Protein Purification -- 2.1.2 Stabilizing Proteins -- 2.1.3 Quantification of Proteins

2.1.4 Purification of Proteins …

Subjects

enzyme Enzymes protein Protein Conformation Protein Folding Protéines Protéines -- Conformation Protéines -- Structure Proteins Proteins -- Conformation Proteins -- Structure