Fundamentals of fluorescence imaging

Fluorescence imaging, at macro, micro, and submicro scales, has revolutionized biological science in the past 30 years. Immunolabelling has provided precise targeting of molecules in fixed tissue, while fluorescent proteins have enabled localization in living tissues. Fluorescent indicators enable imaging of dynamic changes in cell metabolism. This book covers, for the first time, imaging at all scales from macro to submicro (superresolution). Its authors include Robert Clegg, legendary teacher and researcher (who, sadly, passed away during the editing); Jim Pawley, editor of several editions of the Handbook of Biological Confocal Microscopy; the famous and now dispersed New Zealand team of Mark Cannell, Christian Soeller, and David Baddeley; Robert Hoffman, pioneer of whole-animal imaging in cancer research; Andreas Schoenle and Christian Eggeling on STED nanoscopy, and many more famous participants in this field. All the contributors are at the cutting edge of their field.

Chapter 1. Basic Principles of Fluorescence / With Scott Kable -- Chapter 2. The Ploem Fluorescence Microscope / With Guy Cox -- Chapter 3. Confocal Microscopy / With Stephen H. Cody, Guy Cox -- Chapter 4. Multiphoton Microscopy / With Mark B. Cannell, Guy Cox -- Chapter 5. Immunofluorescence / With Anthony F. Henwood -- Chapter 6. Fluorescent Proteins / With Anya Salih -- Chapter 7. Dyes for Labeling Organelles and Compartments / With Iain Johnson -- Chapter 8. Indicator and Reporter Dyes With Iain Johnson -- Chapter 9. New Approaches to Cancer Therapy Using in vivo Fluorescent-Protein Imaging / With Robert M. Hoffman -- Chapter 10. Fluorescence Lifetime Imaging in Living Cells / With Chittanon Buranachai, John P. Eichorst, Kei Wei Teng, Robert M. Clegg -- Chapter 11. Advanced Fluorescence Techniques: FRAP, iFRAP, FLIP, FLAP, Photoconvertible, Photoactivatable, and Photoswitchable Proteins / With Kelly Rogers, Sarah Ellis -- Chapter 12. Förster Resonance Energy Transfer Microscopy for Monitoring Molecular Dynamics in Living Cells / With Vinod Jyothikumar, Yuansheng Sun, Ammasi Periasamy -- Chapter 13. Photodetectors for Fluorescence Microscopy / With James Pawley -- Chapter 14. P…