Galectins: methods and protocols

by Arthur, Connie M. Cummings, Richard D. Stowell, Sean R.

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This second edition is dedicated to new and updated methodological approaches designed to study galectin function. Chapters examine salient features of galectin functions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Galectins: Meth…

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Title Statements

Full title :Galectins : methods and protocols / edited by Sean R. Stowell and Connie M. Arthur, Richard D. Cummings.

Summary

Edition Statements

Publication Date:2022
Edition:Second edition

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:2022

The Physical Object

Description:1 online resource (1 PDF file (xix, 730 pages)) : : illustrations (some color).

Edition Identifiers

ISBN:9781071620557, 107162055X
DOI:10.1007/978-1-0716-2055-7
oclc:on1306588512
LC Classification:QP550
Dewey Decimal:572/.6

Galectins: An Ancient Family of Carbohydrate Binding Proteins with Modern Functions -- Cloning, Expression and Purification of Galectins for In Vitro Studies -- Purification of Recombinant Galectins Using Affinity Chromatography Methods -- Alkylation of Galectin-1 with Iodoacetamide and Mass Spectrometric Mapping of the Sites of Incorporation -- Rapid Detection and Purification of Galectin-3 by the Capture and Release (CaRe) Method -- Introducing 77Se NMR Spectroscopy to Analyzing Galectin-Ligand Interaction -- Evaluation of Galectin Binding by Surface Plasmon Resonance -- Revealing the Identity of Human Galectin-3 as a Glycosaminoglycan-Binding Protein -- Examining Galectin Binding Specificity Using Glycan Microarrays -- Mechanism of Mucin Recognition by Lectins -- A Thermodynamic Study -- Examination of Whole Cell Galectin Binding by Solid Phase and Flow Cytometric Analysis -- Dissecting Context-Specific Galectin Binding Using Glyco-Engineered Cell Libraries -- Method for Identifying Galectin Ligands on Lymphocyte Membrane Glycoproteins -- Transformation of Agrocybe Cylindracea Galectin into aGalNAc-specific Lectin -- What Happens if a Human Galectin Enters the Endoplasmic Retic…