Guidelines for molecular analysis in archive tissues
A huge amount of fixed and paraffin-embedded tissue is stored in every hospital. This is very precious material that can be used for translational research and for diagnostics. The molecular methods employed for analysis of these tissues are similar to the usual molecular biology and proteomics methods, but reliable results can be obtained only if specific steps are followed with great care. ¡ This book provides detailed and precise guidelines for molecular analysis of archive tissues and will …
Read More ↓Title Statements
- Full title :Guidelines for molecular analysis in archive tissues / Giorgio Stanta, editor.
Summary
A huge amount of fixed and paraffin-embedded tissue is stored in every hospital. This is very precious material that can be used for translational research and for diagnostics. The molecular methods employed for analysis of these tissues are similar to the usual molecular biology and proteomics methods, but reliable results can be obtained only if specific steps are followed with great care. ¡ This book provides detailed and precise guidelines for molecular analysis of archive tissues and will serve as an invaluable aid for researchers and pathologists involved in translational research and diagnostics. The topics addressed include pre-analytical treatment of tissues, isolation of tissue components, DNA and RNA methods, proteomics methods, internal quality control procedures, and storage and treatment of reagents. Clear notes and explanations are included to simplify use of the protocols for the less experienced. The authors are a group of acknowledged experts who have developed the described methods and validated them within the European project "Archive Tissues: Improving Molecular Medicine Research and Clinical Practice - IMPACTS", which has involved 21 leading institutions in 11 countries
Read More ↓Edition Statements
- Publication Date:2011.
Publication Statements
- Publisher(s):Springer
- Place of Publication:Heidelberg
- Language:eng
- Publication Date:2011.
The Physical Object
- Description:1 online resource (xvi, 323 pages) : : illustrations (some color)
Edition Identifiers
- ISBN:9783642178900, 3642178901
- DOI:10.1007/978-3-642-17890-0
- oclc:ocn730257085
- LC Classification:RB113
- Dewey Decimal:616.07
Table of Contents
Archive Tissues -- Preanalytical Time Interval (PATI) and Fixation -- Formalin-Free Fixatives -- Manual Microdissection -- Tissue Microarray (TMA) -- Laser Capture Microdissection (LCM) -- DNA Extraction from Formalin-Fixed Paraffin-Embedded (FFPE) Tissues -- DNA Extraction from Formalin-Fixed Paraffin-Embedded Tissues (FFPE) (from Small Fragments of Tissues or Microdissected Cells) -- Fast Protocol for DNA Extraction from Formalin-Fixed Paraffin-Embedded Tissues -- DNA Extraction from Blood and Forensic Samples -- Restoration and Reconstruction of DNA Length -- RNA Extraction from Formalin-Fixed Paraffin-Embedded Tissues -- RNA Extraction from Decalcified and Non-decalcified Formalin-Fixed Paraffin-Embedded Tissues -- RNA Temperature Demodification -- MicroRNA Extraction from Formalin-Fixed Paraffin-Embedded Tissues -- Quantification of Nucleic Acids -- Integrity Assessment of Nucleic Acids -- DNase Treatment of RNA -- Reverse Transcription -- General Protocol for End-Point PCR -- Quantitative End-Point PCR -- Nested-PCR -- General Protocol for Dot-Blot -- PCR-ELISA -- Quantitative Real-Time RT-PCR -- DNA Sequencing from PCR Products -- Pyrosequencing Analysis for Detection of KR…
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