Heterologous expression of membrane proteins: methods and protocols
This detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies. Beginning with techniques using E. coli as a host for the overproduction and purification of membrane proteins, the book continues with chapters covering mammalian membrane protein production in yeast, insect cells, mammalian cells, as well as using virus like particles and acellular systems. Additionally, new detergents and alternatives to detergents allo…
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- Full title :Heterologous expression of membrane proteins : methods and protocols / edited by Isabelle Mus-Veteau.
Summary
This detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies. Beginning with techniques using E. coli as a host for the overproduction and purification of membrane proteins, the book continues with chapters covering mammalian membrane protein production in yeast, insect cells, mammalian cells, as well as using virus like particles and acellular systems. Additionally, new detergents and alternatives to detergents allowing membrane protein purification for structural analyses are described. The book closes with a chapter exploring the use of microscale thermophoresis (MST) to evaluate the binding activity of heterologously expressed proteins directly in crude membrane extracts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Heterologous Expression of Membrane Proteins: Methods and Protocols, Third Edition serves as an ideal guide for scientists aiming to produce and purify functional recombinant membrane proteins for structural studies.
Read More ↓Edition Statements
- Publication Date:2022
- Edition:Third edition
Publication Statements
- Publisher(s):Humana Press
- Place of Publication:New York, NY
- Language:eng
- Publication Date:2022
The Physical Object
- Description:xvii, 467 pages : : illustrations (some color) ; : 26 cm.
Edition Identifiers
- ISBN:9781071623671, 1071623672
- oclc:on1338659085
- LC Classification:QP552.M44
- Dewey Decimal:572.694
Table of Contents
Solubilization and Purification of a5b1 Integrin from Rat Liver for Reconstitution into Nanodiscs -- Membrane Proteins Production in Escherichia coli: Protocols and Rules -- Functional Overexpression of Membrane Proteins in E. coli: The Good, the Bad, and the Ugly -- Heterologous Expression of Membrane Proteins in E. coli -- Overexpression of the ABC Transporter BmrA within Intracellular Caveolµ in Escherichia coli -- Production of Membrane Proteins in Pseudomonas stutzeri -- Overproduction of Membrane-Associated, and Integrated, Proteins Using Saccharomyces cerevisiae -- Purification of Membrane Proteins Overexpressed in Saccharomyces cerevisiae -- Production and Purification of a GFP-Tagged ABC Transporter CaCdr1p -- Membrane Protein Production in the Yeast, P. pastoris -- Production and Preparation of Isotopically-Labelled Human Membrane Proteins in Pichia pastoris for Fast-MAS-NMR Analyses -- Membrane Protein Production in Insect Cells -- Thromboxane A2 G Protein-Coupled Receptor Production and Crystallization for Structure Studies -- Production of Human ABC Transporters and Oligosaccharyltransferase Complexes for Structural Studies -- The Heterologous Expression and Purificat…
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