Membrane analysis

Membrane analysis

by Graham, J. M., (John M.)

Type Book
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Membrane Analysis provides a comprehensive review of laboratory methods for membrane study, with an emphasis on isolating membranes, analysing their composition and architecture, and investigating membrane function.

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Title Statements

  • Full title :Membrane analysis / Joan M. Graham, Joan A. Higgins.

Summary

Membrane Analysis provides a comprehensive review of laboratory methods for membrane study, with an emphasis on isolating membranes, analysing their composition and architecture, and investigating membrane function.

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Edition Statements

  • Publication Date:2006.

Publication Statements

Notes

Originally published: Oxford: Bios Scientific Publishers; New York: Springer, 1997.

The Physical Object

  • Description:1 online resource (224 pages)

Edition Identifiers

  • ISBN:9781003077084, 1003077080, 9781000119695, 1000119696, 9781000141023, 1000141020, 9781000102529, 1000102521
  • oclc:on1190774663
  • LC Classification:QH601
  • Dewey Decimal:571.6/4

Table of Contents

Abbreviations -- Preface -- 1. General membrane composition and structure -- Lipids -- General lipid composition -- Phospholipids -- Isoprenoids -- Lipid architecture -- Proteins -- Peripheral proteins -- Integral proteins -- Lipid-anchored proteins -- Protein architecture in the plane of the membrane -- Carbohydrates -- Glycolipids -- Glycoproteins -- Proteoglycans -- 2. The membranes of prokaryotes and eukaryotes -- General features -- Membranes of prokaryotes -- Membranes of eukaryotes -- The surface (plasma) membrane -- Plasma membrane domains -- The cytoskeleton and glycocalyx -- Mitochondria and chloroplasts -- Peroxisomes (microbodies) -- Lysosomes -- Nuclei -- Endocytic compartment -- The secretory compartment -- References -- 3. Preparation of subcellular membranes -- Homogenization -- Liquid shear -- Gaseous shear -- Mechanical shear -- Other methods -- Homogenization of soft mammalian tissue -- Homogenization of tough tissues -- Homogenization of cultured mammalian cells -- Plant tissue -- Yeast -- Bacteria -- Fractionation of subcellular organelles -- Differential centrifugation -- Density gradient centrifugation -- Density perturbation -- Fractionation by immunoaffini…

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