Histone deacetylases: methods and protocols

by Sarkar, Sibaji

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Title Statements

Full title :Histone deacetylases : methods and protocols / edited by Sibaji Sarkar, Boston University School of Medicine, Boston, MA, USA.

Summary

This volume provides different methodologies for all classes of histone deacetylases, which includes detailed procedures on Class I and II histone deacetylase inhibitors, SIRT inhibitors, and bromodomain inhibitors. Histone Deacetylases: Methods and Protocols is divided into four sections: Sections A and B describe methodologies used to detect the activity, function, or chromatin location of HDACs 1 through 11, with Section A discussing Class I and Section B discussing class II histone deacetylases; Section C focuses on the methodologies for cloning and characterizing the acetylation of SIRTs 1 through 7; and Section D describes methods related to histone deacetylase inhibitors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Histone Deacetylases: Methods and Protocols, is a valuable resource for investigators working on epigenetics, molecular biology, and genetics.

Edition Statements

Publication Date:2016

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York
Language:eng
Publication Date:2016

The Physical Object

Description:xii, 337 pages : : illustrations (some color) ; : 26 cm

Edition Identifiers

ISBN:9781493936656, 1493936654
oclc:ocn936532711
LC Classification:QP552.H5
Dewey Decimal:572/.66

1. A Sensitive and Flexible Assay for Determining Histone Deacetylase 1 (HDAC1) Activity -- 2. Detection of Sumo Modification of Endogenous Histone Deacetylase 2 (HDAC2) in Mammalian Cells -- 3. Analysis of Epigenetic Regulation of Hypoxia-Induced Epithelial-Mesenchymal Transition in Cancer Cells by Quantitative Chromatin Immunoprecipitation of Histone Deacetylase 3 (HDAC3) -- 4. Molecular and Functional Characterization of Histone Deacetylase 4 (HDAC4) -- 5. Approaches for Studying the Subcellular Localization, Interactions, and Regulation of Histone Deacetylase 5 (HDAC5) -- 6. Analysis of Expression and Functions of Histone Deacetylase 6 (HDAC6) -- 7. Analysis of Histone Deacetylase 7 (HDAC7) Alternative Splicing and Its Role in Embryonic Stem Cell Differentiation Toward Smooth Muscle Lineage -- 8. Large-Scale Overproduction and Purification of Recombinant Histone Deacetylase 8 (HDAC8) from the Human-Pathogenic Flatworm Schistosoma mansoni -- 9. Visualization of HDAC9 Spatiotemporal Subcellular Localization in Primary Neuron Cultures -- 10. Expression and Function of Histone Deacetylase 10 (HDAC10) in B Cell Malignancies -- 11. Functional Analysis of Histone Deacetylase 11 (HDAC…

Subjects

Group III Histone Deacetylases Histone deacetylase Histone Deacetylase Inhibitors Histone Deacetylases Histone désacétylase Laboratory Manual Laboratory manuals Manuels de laboratoire