HIV Protocols

by Kalpana, Ganjam V. Prasad, Vinayaka R.

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Title Statements

Full title :HIV Protocols / edited by Vinayaka R. Prasad, Ganjam V. Kalpana.

Summary

This third edition volume expands upon the previous editions with new research topics and numerous leading-edge HIV protocols. This book contains chapters that cover various aspects of HIV-1 replication and are helpful for investigating the mechanisms of HIV-1 replication or pathogenesis. The chapters are grouped into six sections: HIV early events; HIV-1 RNA structure and RNA-protein interactions; HIV-1 post-integration events; HIV-1 pathogenesis in animal models; tools to study HIV-1 latency; and NeuroAIDS. Chapters are written by research experts who have diligently developed techniques, improved them, simplified them, or applied them to their own HIV research. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, HIV Protocols, Third Edition is a great resource for HIV research, and contains many significant tips and tricks rarely mentioned in standard protocols.

Edition Statements

Publication Date:2016.
Edition:3rd ed. 2016

Publication Statements

Publisher(s):Humana Press, Imprint, Springer New York
Place of Publication:New York, NY
Language:eng
Publication Date:2016.

Notes

Bibliographic Level Mode of Issuance: Monograph.

The Physical Object

Description:1 online resource (XIV, 383 pages 63 illustrations, 36 illustrations in color.)

Edition Identifiers

ISBN:149393046X, 9781493930463, 9781493930456, 1493930451, 9781493930470, 1493930478, 9781493979790, 1493979795
DOI:10.1007/978-1-4939-3046-3.
oclc:on1109231198
LC Classification:QR180-189.5
Dewey Decimal:616.079

Quantifying CD4/CCR5 Usage Efficiency of HIV-1 ENV Using the Affinofile System -- Measuring T Cell-to-T Cell HIV-1 Transfer, Viral Fusion, and Infection Using Flow Cytometry -- HIV-1 Capsid Stabilization Assay -- Detection and Tracking of Dual-Labeled HIV Particles Using Wide-Field Live Cell Imaging to Follow Viral Core Integrity -- HIV-1 Reverse Transcriptase Based Assay to Determine Cellular dNTP Concentrations -- Rapid Determination of HIV-1 Mutant Frequencies and Mutation Spectra Using an mCherry/EGFP Dual Reporter Viral Vector -- Novel Biochemical Tools for Probing HIV RNA Structure -- Analysis of HIV-1 Gag-RNA Interactions in Cells and Virions by CLIP-seq -- Isolation of Cognate Cellular and Viral Ribonucleoprotein Complexes of HIV-1 RNA Applicable to Proteomic Discovery and Molecular Investigations -- Methods for the Analyses of Inhibitor-Induced Aberrant Multimerization of HIV-1 Integrase -- Quantification of HIV-1 Gag Localization Within Virus Producer Cells -- Methods to Study Determinants for Membrane Targeting of HIV-1 Gag In Vitro -- Visualizing the Behavior of HIV-Infected T Cells In Vivo Using Multiphoton Intravital Microscopy -- Modeling HIV-1 Mucosal Transmission …

Subjects

Allergy and Immunology Analytical, Diagnostic and Therapeutic … biochemistry Biochimie Biological Science Disciplines biological sciences Biologie biology Chemistry Chimie Disciplines and Occupations Genetics HIV HIV (Viruses) Immunologic Techniques Immunologie Immunology Investigative Techniques Lentivirus Lentivirus de primates