Intermediate filament associated proteins

by Sonnenberg, Arnoud Wilson, Katherine L.

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Intermediate Filament Associated Proteins, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in intermediate filament associated proteins and contains sections on such topics as lamin-associated proteins, intermediate filament-associated proteins and plakin, and other cytoskeletal cross-linkers.

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Title Statements

Full title :Intermediate filament associated proteins / edited by Katherine L. Wilson, Arnoud Sonnenberg.

Summary

Edition Statements

Publication Date:2016.
Edition:First edition

Publication Statements

Publisher(s):Academic Press, an imprint of Elsevier
Place of Publication:Waltham, MA
Language:eng
Publication Date:2016.

The Physical Object

Description:1 online resource : : illustrations (chiefly color)

Edition Identifiers

ISBN:9780128034903, 0128034904, 0128034696, 9780128034699
oclc:ocn935112541
LC Classification:QD431
Dewey Decimal:572.6

Front Cover -- Intermediate Filament Associated Proteins -- Copyright -- Contents -- Contributors -- Preface -- Part I: Identification and Biochemical Analysis of Lamin-Associated Proteins -- Chapter One: BioID Identification of Lamin-Associated Proteins -- 1. Introduction -- 1.1. Nuclear Lamina -- 1.2. Protein-Protein Interactions -- 1.3. BioID -- 2. BioID Method -- 2.1. Construction of a BioID-Fusion Protein -- 2.2. Cell Choice -- 2.3. Functional Assessment of the Fusion Protein -- 2.4. Establishing a Stable BioID Cell Line -- 2.4.1. Materials.

2.4.2. Generation and Validation of BioID-Fusion Protein -- 2.4.3. Generate Cells That Stably Express the BioID-Fusion Protein -- 2.5. Large-Scale BioID Pull-Down to Identify Interacting Proteins -- 2.5.1. Materials -- 2.5.2. Cell Lysis -- 2.5.3. Affinity Purification of Biotinylated Proteins -- 2.6. Reagents and Solutions -- 3. Anticipated Results -- 4. Conclusions -- Acknowledgment -- References -- Chapter Two: Proximity-Dependent Biotin Identification (BioID) in Dictyostelium Amoebae -- 1. Introduction -- 1.1. Lamins and Their Partners: The Challenge -- 1.2. Advantages and Limitations of BioID.

1.2.1. Limitations of BioID -- 1.3. Ada…