Intermediate filament proteins

Type Book

Rate 0.0 /5

Intermediate Filament Proteins, the latest volume in the Methods in Enzymology series covers all the intermediate filaments in vertebrates and invertebrates, providing a unique understanding of the multiple different tissue-specific intermediate filaments. This volume also covers the latest methods that are currently being used to study intermediate filament protein function and dynamics. It will be an important companion for any experimentalist interesting in studying this protein family in th…

Details

Files

Reviews

Download

Title Statements

Full title :Intermediate filament proteins / edited by M. Bishr Omary and Ronald K.H. Liem.

Summary

Edition Statements

Publication Date:©2016

Publication Statements

Publisher(s):Academic Press, an imprint of Elsevier
Place of Publication:Waltham, MA
Language:eng
Publication Date:©2016

The Physical Object

Description:1 online resource (xxv, 791 pages) : : illustrations (chiefly color)

Edition Identifiers

ISBN:9780128034910, 0128034912
oclc:ocn935638376
LC Classification:QD431
Dewey Decimal:572/.67

Front Cover; Intermediate Filament Proteins; Copyright; Dedication; Contents; Contributors; Preface; References; Acknowledgments; Part I: General Methods to Study IF Proteins; Chapter One: How to Study Intermediate Filaments in Atomic Detail; 1. Introductory Remarks; 2. Bioinformatics; 3. X-ray Crystallography; 3.1. Design of IF Protein Fragments for Crystallization; 3.2. Protein Expression; 3.3. Purification; 3.4. Crystallization; 3.5. X-ray Data Collection; 3.6. Phasing by Molecular Replacement; 3.7. Experimental Phasing Using Heavy Atoms; 3.8. Structure Refinement and Validation.

3.9. Limitations and Pitfalls3.10. Obtained Structures and Impact; 4. Electron Paramagnetic Resonance with Site-Directed Spin Labeling; 4.1. Principle; 4.2. Sample Preparation and Measurements; 4.3. Data Interpretation and Impact; 4.4. Limitations and Outlook; 5. Concluding Remarks; Acknowledgments; References; Chapter Two: Mechanical Properties of Intermediate Filament Proteins; 1. Introduction; 2. Viscoelasticity of Purified IFs In Vitro; 3. IFs and the Mechanical Properties of Cells; 3.1. Type III IF: Vimentin and Desmin; 3.1.1. Knockout Models of Vimentin.

3.1.2. Drugs and Proteins Disrupting Vim…