Laboratory methods in enzymology

Type Book

Rate 0.0 /5

In this volume we have brought together a number of core protocols concentrating on Protein, carefully written and edited by experts.

Details

Files

Reviews

Download

Title Statements

Full title :Laboratory methods in enzymology. / edited by Jon R. Lorsch.

Summary

In this volume we have brought together a number of core protocols concentrating on Protein, carefully written and edited by experts.

Edition Statements

Publication Date:©2015
Edition:First edition

Publication Statements

Publisher(s):Elsevier Academic Press
Place of Publication:London
Language:eng
Publication Date:©2015

The Physical Object

Description:1 online resource (xviii, 470 pages) : : color illustrations

Edition Identifiers

ISBN:9780128003350, 0128003359
oclc:ocn914434179
LC Classification:QP601
Dewey Decimal:572/.6

Front Cover; Laboratory Methods in Enzymology: Protein Part D; Copyright; Contents; Contributors; Preface; Chapter One: Purification of His-Tagged Proteins; 1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions & buffers; 4. Protocol; 4.1. Preparation; 4.2. Duration; 5. Protocol A: Purification of His-tagged Proteins Under Native Conditions; 5.1. Tip; 6. Step 1A Preparation of a Cleared E. coli Lysate Under Native Conditions; 6.1. Overview; 6.2. Duration; 6.3. Tip; 6.4. Tip; 6.5. Tip; 6.6. Tip; 6.7. Tip; 7. Step 2A Batch Purification of His-tagged Proteins from E. coli Under Native Conditions.

7.1. Overview7.2. Duration; 7.3. Tip; 7.4. Tip; 7.5. Tip; 7.6. Tip; 8. Step 1B Preparation of a Cleared E. coli Lysate Under Denaturing Conditions; 8.1. Overview; 8.2. Duration; 8.3. Tip; 8.4. Tip; 8.5. Tip; 8.6. Tip; 8.7. Tip; 9. Step 2B Batch Purification of His-tagged Proteins from E. coli Under Denaturing Conditions; 9.1. Overview; 9.2. Duration; 9.3. Tip; 9.4. Tip; 9.5. Tip; 9.6. Tip; References; Referenced Literature; Source References; Referenced Protocols in Methods Navigator.

Chapter Two: Affinity Purification of a Recombinant Protein Expressed as a Fusion with the Maltose-Binding…