Lectins: Methods and Protocols

by Hirabayashi, Jun

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Title Statements

Full title :Lectins : Methods and Protocols / edited by Jun Hirabayashi.

Summary

Lectins: Methods and Protocols summarizes classic lectin technologies and advanced techniques with high throughputs and sensitivities. Chapters include methods and techniques for serial lectin-affinity chromatography procedure, lectin-probed western blot and histochemical analyses, quantitative interaction analyses based on equilibrium dialysis, isothermal calorimetry, surface plasmon resonance, techniques for elucidating functions of endogenous animal lectins, advanced methods on "engineer" novel lectins by evolutionary concepts, and special equipment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Lectins: Methods and Protocols seeks to aid not only lectin specialists but also non-experts including both young scientists

Edition Statements

Publication Date:2014.

Publication Statements

Publisher(s):Humana Press, Imprint, Springer New York
Place of Publication:New York, NY
Language:eng
Publication Date:2014.

Notes

Bibliographic Level Mode of Issuance: Monograph.

The Physical Object

Description:1 online resource (XIII, 613 pages 168 illustrations, 60 illustrations in color.)

Edition Identifiers

ISBN:1493912925, 9781493912926, 9781493912919, 1493912917
DOI:10.1007/978-1-4939-1292-6.
oclc:on1167880814
LC Classification:QD431-431.7
Dewey Decimal:572/.69

History of Plant Lectin Research -- Fungal Lectins a Growing Family -- The "White Kidney Bean Incident" in Japan -- Hemagglutination (Inhibition) Assay -- Preparation of Affinity Adsorbents and Purification of Lectins from Natural Sources -- High-Performance Lectin Affinity Chromatography -- Determination of Glycan Motifs Using Serial Lectin Affinity Chromatography -- Lectin-Probed Western Blot Analysis -- Solid-Phase Assay of Lectin Activity Using HRP-conjugated Glycoproteins -- Sialidase-dependent Binding Assay for Influenza and Related Viruses -- Lectin Affinity Electrophoresis -- Capillary-Based Lectin Affinity Electrophoresis for Interaction Analysis between Lectins and Glycans -- Basic Procedures for Lectin Flow Cytometry -- Histochemical Staining Using Lectin Probes -- Equilibrium Dialysis using Chromophoric Sugar Derivatives -- Centrifugal Ultrafiltration-HPLC Method for Interaction Analysis between Lectins and Sugars -- Surface Plasmon Resonance as a Tool to Characterize Lectin-carbohydrate Interactions -- Isothermal Calorimetric Analysis of Lectin-sugar Interaction -- Carbohydrate-Lectin Interaction Assay by Fluorescence Correlation Spectroscopy Using Fluorescence-Labele…

Subjects

biochemistry biological sciences Biology, life sciences lectin technologies lectin-probed western blot Lectins Life Sciences Mathematics and science protein Protéines Proteins Proteomics Protéomique Sciences de la vie serial lectin-affinity chromatography