Lipid-protein interactions: methods and protocols

by Kleinschmidt, Jörg H.

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Title Statements

Full title :Lipid-protein interactions : methods and protocols / edited by Jörg H. Kleinschmidt.

Summary

This second edition volume expands all chapters of the previous edition, which have been enhanced to cover the most recent developments, the current state of method research, and applications. Additional protocols were added to examine lipid-protein interactions by mass spectrometry, to use protein microarrays to investigate large sets of various proteins, to study membrane protein dynamics by UV resonance Raman spectroscopy, to analyze peptide-induced pore formation in membranes, and to investigate folding and insertion of membrane proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Lipid-Protein Interactions: Methods and Protocols, Second Edition is an essential resource for all researchers who are interested in obtaining up-to-date and comprehensive information about membrane structure and function.

Edition Statements

Publication Date:2019
Edition:Second edition

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:2019

The Physical Object

Description:xiv, 682 pages : : illustrations ; : 27 cm.

Edition Identifiers

ISBN:9781493995110, 1493995111, 149399512X, 9781493995127
oclc:on1112386391
Dewey Decimal:572.57

Multiscale Modeling and Simulation Approaches to Lipid-Protein Interactions -- Quartz Crystal Microbalances as Tools for Probing Protein-Membrane Interactions -- Surface Plasmon Resonance for Measuring Interactions of Proteins with Lipids and Lipid Membranes -- Thermodynamic Analysis of Protein-Lipid Interactions by Isothermal Titration Calorimetry -- Differential Scanning Calorimetry of Protein-Lipid Interactions -- Imaging and Force Spectroscopy of Single Transmembrane Proteins with the Atomic Force Microscope -- Kinetics of Insertion and Folding of Outer Membrane Proteins by Gel-Electrophoresis -- Optimized Negative-Staining Protocol for Lipid-Protein Interactions Investigated by Electron Microscopy -- Probing Heterogeneous Lipid Interactions with Membrane Proteins using Mass Spectrometry -- Protein Microarrays and Liposome: A Method for Studying Lipid-Protein Interactions -- Structural Investigations of Protein-Lipid Complexes using Neutron Scattering -- Circular-Dichroism and Synchrotron-Radiation Circular-Dichroism Spectroscopy as Tools to Monitor Protein Structure in a Lipid Environment -- FTIR Analysis of Proteins and Protein-Membrane Interactions -- UV Resonance Raman Spe…

Subjects

biochemistry Biochimie Carrier Proteins Laboratory Manual Laboratory manuals lipid Lipid membranes Lipides lipids Lipids -- chemistry Manuels de laboratoire mass spectrometry Membrane Lipids -- chemistry Membrane Proteins -- chemistry Protéines de liaison Proteins -- chemistry Spectrométrie de masse