Mass spectrometry-based lipidomics: methods and protocols

by Hsu, Fong Fu

Type Book

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Title Statements

Full title :Mass spectrometry-based lipidomics : methods and protocols / edited by Fong-Fu Hsu.

Summary

This detailed volume covers conventional MS-based "shotgun lipidomics" by which samples are introduced by infusion or loop injection, as well as LC-MS-based lipidomics, which are becoming increasingly important due to the ever-increasing demand for a complete and precise lipid analysis of the complex and diversified lipids in nature. The volume features protocols applying chemical reactions, the on-line photochemical reactions combined with various MS methods for comprehensive characterization of various lipid classes, and quantification of specific and rare lipids. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mass Spectrometry-Based Lipidomics: Methods and Protocols serves as an invaluable guide for biochemists and mass spectroscopists who are interested in lipid studies

Edition Statements

Publication Date:2021

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:2021

Notes

Includes index.

The Physical Object

Description:1 online resource (xi, 314 pages) : : illustrations (some color)

Edition Identifiers

ISBN:9781071614105, 107161410X
DOI:10.1007/978-1-0716-1410-5
oclc:on1250315116
LC Classification:QP751
Dewey Decimal:572/.57

Mass Spectrometry-Based Lipidomics: An Overview -- High Confidence Shotgun Lipidomics Using Structurally Selective Ion Mobility-Mass Spectrometry -- Global Lipidomics Profiling by a High Resolution LC-MS Platform -- Comprehensive Structural Characterization of Lipids by Coupling Paternò-Büchi Reaction and Tandem Mass Spectrometry -- Chemical Derivatization-Aided High Resolution Mass Spectrometry for Shotgun Lipidome Analysis -- Quantitative Analysis of Polyphosphoinositide, Bis(monoacylglycero)phosphate, and Phosphatidylglycerol Species by Shotgun Lipidomics after Methylation -- A Mass Spectrometry-Based Shotgun Lipidomics Using Charge-Switch Derivatization for Analysis of the Complex Long-Chain Fatty Acids -- One-Pot Extractive Transesterification of Fatty Acids Followed by DMOX Derivatization for Location of Double Bonds Using GC-EI-MS -- Ceramide Analysis by Multiple Linked-Scan Mass Spectrometry Using a Tandem Quadrupole Instrument -- Comprehensive Mouse Skin Ceramide Analysis on a Solid-Phase and TLC Separation with High Resolution Mass Spectrometry Platform -- Mass Spectrometric Analysis of Meibomian Gland Lipids -- Quantification of Plasma Oxylipins Using Solid Phase Extrac…

Subjects

Electronic books Laboratory Manual Laboratory manuals Lipides -- Manuels de laboratoire. -- A… Lipids -- analysis Lipids -- Laboratory manuals. -- Analys… Manuels de laboratoire mass spectrometry Mass spectrometry -- Laboratory manuals Spectrométrie de masse -- Manuels de la…