Melanoma: methods and protocols

by Hargadon, Kristian M., (Kristian Michael)

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Title Statements

Full title :Melanoma : methods and protocols / edited by Kristian M. Hargadon.

Summary

This extensive book brings together leading melanoma researchers from across the world and highlights many of the cutting-edge protocols and experimental systems currently being used to investigate questions surrounding this disease. The volume opens with sections on 2D and 3D cell culture-based approaches for studying melanoma biology, and continues with collections of chapters examining various approaches for detecting, isolating, and characterizing circulating melanoma cells, circulating tumor DNA, and exosomes, as well as experimental procedures for studying and detecting melanoma metastasis in both pre-clinical and clinical settings, bioinformatics-based approaches, protocols for quantifying and characterizing immune cell infiltrates in both melanoma tumors and tertiary lymphoid structures, and development and evaluation of therapeutic strategies for melanoma treatment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Melanoma: Methods and Protocols aims to serve basic research scientists and clinicians who bring questions from the clinic into the lab in order to translate observations in the laboratory into improved patient care for this highly malignant form of cancer. Chapter 14 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Edition Statements

Publication Date:2021

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York
Language:eng
Publication Date:2021

The Physical Object

Description:xx, 674 pages : : illustrations (some color) ; : 26 cm.

Edition Identifiers

ISBN:9781071612040, 1071612042
oclc:on1193114465
LC Classification:RC280.M37
Dewey Decimal:616.99477

Model Systems for the Study of Malignant Melanoma -- Generation of Functional Gene Knockout Melanoma Cell Lines by CRISPR-Cas9 Gene Editing -- A Fluorescent Gelatin Degradation Assay to Study Melanoma Breakdown of Extracellular Matrix -- Wound Healing Assay for Melanoma Cell Migration -- A Fluorescence-Based Assay for Measuring Glucose Uptake in Living Melanoma Cells -- Analyzing Melanoma Cell Oxygen Consumption and Extracellular Acidification Rates Using Seahorse Technology -- Analysis of Melanoma Cell Glutamine Metabolism by Stable Isotope Tracing and Gas Chromatography-Mass Spectrometry -- Determination of Cytotoxic Activities Against Melanoma Cells Using Flow Cytometry -- In Vitro Differentiation of Tumor-Associated Macrophages from Monocyte Precursors with Modified Melanoma-Conditioned Medium -- A Flow Cytometric Assay for Investigating Melanoma Cell Adhesion to Lymphatic Endothelial Cells -- An Approach to Study Melanoma Invasion and Crosstalk with Lymphatic Endothelial Cell Spheroids in 3D Using Immunofluorescence -- Evaluating Melanoma Viability and Proliferation in 3D Microenvironments -- Preparation, Drug Treatment, and Immunohistological Analysis of Tri-Culture Spheroid…