Membrane proteins: Production and functional characterization

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Membrane Proteins - Production and Function Characterization a volume of Methods in Enzymology, encompasses chapters from the leading experts in the area of membrane protein biology. The chapters provide a brief overview of the topics covered and also outline step-by-step protocol. Illustrations and case example images are included wherever appropriate to help the readers understand the schematics and general experimental outlines.

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Title Statements

Full title :Membrane proteins : Production and functional characterization / edited by Arun K. Shukla.

Summary

Edition Statements

Publication Date:©2015

Publication Statements

Publisher(s):Elsevier Academic Press
Place of Publication:Amsterdam, Netherlands
Language:eng
Publication Date:©2015

The Physical Object

Description:1 online resource (xxiv, 651 pages and 14 pages of plates) : : illustrations (some color)

Edition Identifiers

ISBN:0128016256, 9780128016251
oclc:ocn907479210
LC Classification:QP552.M44
Dewey Decimal:572/.696

Engineering Escherichia coli for functional expression of membrane proteins -- ACEMBLing a multiprotein transmembrane complex: the functional SecYEG-SecDF-YajC-YidC holotranslocon protein secretase/insertase -- Expression of purification of OsVDAC4 -- Membrane protein expression in Lactococcus lactis -- An unconventional anaerobic membrane protein production system based on Wolinella succinogenes -- Membrane protein expression and analysis in yeast -- Heterologous expression of G-protein-coupled receptors in yeast -- Recombinant G protein-coupled receptor expression in Saccharomyces cerevisiae for protein characterization -- Baculovirus-mediated expression of GPCRs in insect cells -- Expression of membrane proteins in the eyes of transgenic Drosophila melangaster -- Rapid method to express and purify human membrane protein using the Xenopus Oocyte system for functional and low-resolution structural anlaysis -- Expression of G protein-coupled receptors in mammalian cells -- Construction of stable mammalian cell lines for inducible expression of G protein-coupled receptors -- Rapid and facile recombinant expression of bovine rhodopsin in HEK293S GnTI- cells using a PiggyBac inducibl…